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Ferbian M. Siswanto, Ferbian M.
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Biochemistry, Genetics & Molecular Biology Health Professions

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Pemberian resveratrol oral mencegah peningkatan F2-Isoprostan urin tikus Wistar (Rattus norvegicus) jantan yang dipapar tartrazine ., Yulyani; Pangkahila, Wimpie; Siswanto, Ferbian M.
Jurnal Biomedik : JBM Vol 9, No 1 (2017): JURNAL BIOMEDIK : JBM
Publisher : UNIVERSITAS SAM RATULANGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35790/jbm.9.1.2017.15316

Abstract

Abstract: This study was aimed to prove that oral administration of resveratrol could prevent urinary F2-isoprostane elevation in tartrazine-induced male Wistar rats (Rattus norvegicus). This was an experimental study using the pretest-posttest control group design. Subjects were 24 rats (Rattus norvegicus), Wistar strain, healthy, 2-3 months old, weighing 200-220 g, divided into 2 groups with 12 rats each. The first group (P0), the control group, was given a placebo of 2 ml distilled water 2 hours prior to the administration of tartrazine 75 mg/kg body weight. The second group (P1), the treatment group, was given resveratrol of 20 mg/kg 2 hour prior to the administration of tartrazine 75 mg/kg. Rats’ urine was collected before and after treatment for 4 weeks. Level of F2-isoprostane was examined by using an 8-iso-PGF2α enzyme immuno assay kit. The comparative analysis of the pretest groups showed that there was no difference between the average levels of F2-isoprostane in both groups (5.45±0.62 ng/mL in P0 group vs 5.42±0.64 ng/mL in P1 group) (P > 0.05). Meanwhile, after treatment for 4 weeks, the average level of F2-isoprostane in the P0 group was significantly different from the P1 group (6.61±0.93 ng/mL vs 3.79±0.48 ng/mL) (P < 0.01). Analysis of the treatment effect showed a significant increase of F2-isoprostane level in the P0 group, and a significant decrease in the P1 group (P < 0.01). Conclusion: Oral administration of resveratrol could prevent urinary F2-isoprostane elevation in tartrazine-induced male Wistar rats (Rattus norvegicus).Keywords: tartrazine, resveratrol, F2-isoprostaneAbstrak: Tujuan penelitian untuk membuktikan pemberian resveratrol oral dapat mencegah peningkatan F2-isoprostan dalam urin tikus (Rattus norvegicus) Wistar jantan yang dipapar tartrazine. Jenis penelitian eksperimental dengan menggunakan pretest-posttest control group design. Subyek penelitian ialah 24 ekor tikus (Rattus norvegicus), galur Wistar, sehat, berumur 2-3 bulan, dengan berat badan 200-220 gr, dibagi menjadi 2 kelompok, masing-masing berjumlah 12 ekor tikus. Kelompok pertama (P0) ialah kelompok kontrol, diberikan tartrazine 75 mg/kg BB dan 2 jam setelahnya diberikan plasebo berupa aquadest 2 ml. Kelompok kedua (P1) ialah kelompok perlakuan, diberikan tartrazine 75 mg/kg BB dan 2 jam setelahnya diberikan resveratrol 20 mg/kg BB. Saat sebelum dan sesudah perlakuan selama 4 minggu, urin tikus dikoleksi untuk pemeriksaan kadar F2-isoprostan menggunakan 8-iso-PGF2α enzyme immuno assay kit. Analisis komparasi sebelum perlakuan (pretest) menunjukkan rerata kadar F2-isoprostan pada kedua kelompok tidak berbeda nyata (5,45±0,62 ng/mL vs 5,42±0,64 ng/mL) (P > 0,05). Setelah perlakuan selama 4 minggu, rerata kadar F2-isoprostan pada kelompok P0 berbeda nyata dibandingkan kelompok P1 (6,61±0,93 ng/mL vs 3,79±0,48 ng/mL) (P < 0,01). Analisis efek perlakuan menunjukkan terjadi peningkatan kadar F2-isoprostan pada kelompok P0 dan penurunan bermakna pada kelompok P1 (P < 0,01). Simpulan: Pemberian resveratrol oral dapat mencegah peningkatan F2-isoprostan urin tikus (Rattus norvegicus) Wistar jantan yang dipapar tartrazine.Kata kunci: tartrazine, resveratrol, F2-isoprostan, urin
Pelatihan Fisik Berlebih Meningkatkan Indeks Apoptosis pada Hepatosit Tikus (Rattus norvegicus) Wistar Jantan Pangkahila, Elionardo; Linawati, Ni M.; Sugiritama, I. W.; Siswanto, Ferbian M.
Jurnal Biomedik : JBM Vol 11, No 3 (2019): JURNAL BIOMEDIK : JBM
Publisher : UNIVERSITAS SAM RATULANGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35790/jbm.11.3.2019.26308

Abstract

Abstract: Excessive physical training or overtraining can increase the production of free radicals such as peroxide, superoxide, and hydroxyl radicals. These free radicals can cause apoptosis of various organ cells, including liver. This study was aimed to prove that overtraining led to hepatocyte apoptosis in male Wistar rats (Rattus norvegicus). This was an experimental laboratory study. Samples were 16 healthy male Wistar rats, aged 2.5-3 months, weighing ±200 grams, which were divided into 2 groups (n= 8 rats), as follows: control group (P0) and treatment group (P1). The control group was not given any treatment (sedentary lifestyle) meanwhile the treatment group was given overtraining (60 minutes of swimming, 7 times a week for 3 weeks). After 3 weeks of treatment, all rats were euthanized with ketamine to obtain the liver specimens. The apoptotic index was examined in histopathological slides with HE staining, 3 fields of views each, at 400 times magnification. The results showed that excessive physical training increased the apoptotic index in hepatocyte cells by ~700%. The apoptosis index in the P0 group was 0.78±0.31%, whereas in the P1 group was 5.42±0.44% (p<0.001). In conclusion, overtraining leads to apoptosis in the hepatocytes of male Wistar rats.Keywords: overtraining, apoptosis, hepatocytes Abstrak: Pelatihan fisik berlebih dapat meningkatkan produksi radikal bebas seperti radikal peroksida, superoksida, dan hidroksil. Radikal bebas ini dapat berlanjut menyebabkan apoptosis berbagai sel-sel organ, termasuk hati. Penelitian ini bertujuan untuk membuktikan bahwa pelatihan fisik berlebih dapat meningkatkan indeks apoptosis pada hepatosit tikus (Rattus norvegicus) Wistar jantan. Pada penelitian ini digunakan 16 ekor tikus wistar jantan berumur 2,5-3 bulan dengan berat badan ±200 gram dan sehat, dibagi menjadi 2 kelompok (n= 8 ekor) yaitu kelompok kontrol (P) dan perlakuan (P1). Kelompok kontrol tidak diberikan perlakuan sedangkan kelompok perlakuan diberi pelatihan berlebih. Setelah 21 hari perlakuan, tikus dieuthanasi dengan menggunakan ketamin untuk diambil organ hati. Indeks apoptosis diperiksa pada sediaan histopatologik dengan pewarnaan Hematoksilin Eosin (HE) pada 3 lapang pandang pembesaran 400x. Hasil penelitian menunjukkan bahwa pelatihan fisik berlebih meningkatkan indeks apoptosis pada hepatosit sebesar ~700%. Indeks apoptosis pada kelompok P0 ialah 0,78±0,31%, sedangkan pada kelompok P1 ialah 5,42±0,44% (p<0,001). Simpulan penelitian ini ialah pelatihan fisik berlebih meningkatkan indeks apoptosis pada hepatosit tikus Wistar jantan.Kata kunci: pelatihan fisik berlebih, apoptosis, hepatosit
Pelatihan Fisik Berlebih Menurunkan Jumlah Hematopoietic Stem Cells (HSCs) Dibandingkan Pelatihan Fisik Seimbang pada Tikus (Rattus norvegicus) Wistar Jantan ., Zenitalia; Pangkahila, Alex; Pangkahila, Wimpie; Siswanto, Ferbian M.
Jurnal Biomedik : JBM Vol 10, No 1 (2018): JURNAL BIOMEDIK : JBM
Publisher : UNIVERSITAS SAM RATULANGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35790/jbm.10.1.2018.18996

Abstract

Abstract: This study was aimed to prove that balanced physical exercise increased mobilization of hematopoietic stem cells (HSCs) compared to excessive physical exercise in male Wistar rats (Rattus norvegicus). This was an experimental study with the randomized pretest-posttest control group design. Subjects were 24 male Wistar rats (Rattus norvegicus), 2.5-3 months old, weighing 180-200 g, healthy (active and did not show any anatomical abnormality), divided into two groups of 12 rats each. One group (P0) was treated with balanced physical exercise and the other group (P1) was treated with excessive physical exercise. Before (pretest) and 4 weeks after treatment (posttest), peripheral blood of 1 ml was drawn through medial canthus sinus orbitalis for examination of HSCs number in peripheral blood quantitatively. The results showed that the number of HSCs in the P0 group was increased from 1.60±0.70 x 106 cell/μl before treatment to 2.70±0.62 x 106 cells/μl after 4-week treatment (P <0.05). Meanwhile, in the P1 group, the number of HSCs was decreased from 1.74±0.68 x 106 cells/μl before treatment to 1.34±0.55 x 106 cells/μl after 4-week treatment (P <0.05). Conclusion: Excessive physical exercise decreased number of hematopoietic stem cells (HSCs) compared to balanced physical exercise in male Wistar rats (Rattus norvegicus).Keywords: balanced physical exercise, excessive physical exercise, hematopoietic stem cells (HSCs)Abstrak: Tujuan penelitian ini ialah untuk membuktikan bahwa pelatihan fisik berlebih menurunkan jumlah hematopoietic stem cells (HSCs) dibandingkan pelatihan fisik seimbang pada tikus (Rattus norvegicus) Wistar jantan. Jenis penelitian ini ialah eksperimental dengan randomized pre-post test control group design. Subyek penelitian ialah 24 ekor tikus Wistar (Rattus norvegicus) jantan, umur 2,5-3 bulan, berat badan 180-200 gr, sehat (aktif dan tidak menunjukkan abnormalitas anatomi) yang dibagi menjadi dua kelompok yaitu P0 dan P1, masing-masing berjumlah 12 ekor. Kelompok P0 diberikan pelatihan seimbang dan kelompok P1 diberi pelatihan fisik berlebih. Sebelum (pretest) dan 4 minggu setelah perlakuan (posttest), darah diambil sebanyak 1 ml melalui medial canthus sinus orbitalis untuk pemeriksaan kadar HSCs darah tepi secara kuantitatif. Hasil penelitian menunjukkan bahwa pada kelompok P0 terjadi peningkatan jumlah HSCs dari 1,60±0,70 x106 sel/μl pretest menjadi 2,70±0,62 x106 sel/μl posttest (P <0,05). Pada kelompok P1 terjadi penurunan jumlah HSCs dari 1,74±0,68 x106 sel/μl pretest menjadi 1,34±0,55 x106 sel/μl posttest (P <0,05). Simpulan: Pelatihan fisik berlebih menurunkan jumlah hematopoietic stem cells (HSCs) dibandingkan pelatihan fisik seimbang pada tikus (Rattus norvegicus) Wistar jantan.Kata kunci: pelatihan fisik seimbang, pelatihan fisik berlebih, hematopoietic stem cells (HSCs)
AKTIVITAS FISIK MAKSIMAL AKUT (ACUTE OVERTRAINING) MENYEBABKAN KERUSAKAN SEL β PANKREAS MENCIT Siswanto, Ferbian M.; Yenniastuti, Boedi P.; Putra, Tri A.; Kardena, I Made
Jurnal Biomedik : JBM Vol 7, No 2 (2015): JURNAL BIOMEDIK : JBM
Publisher : UNIVERSITAS SAM RATULANGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35790/jbm.7.2.2015.9328

Abstract

Abstract: Excessive physical activity can cause oxidative stress characterized by increased free radicals in the body. This oxidative stress will cause damages to a variety of cells, inter alia β cells of islets of Langerhans in pancreas. This study aimed to determine that acute overtraining could increase the risk of type I diabetes mellitus through induction of pancreatic β cell damages. This study used a completely randomized design with total samples of 36 mice divided into 3 groups. Group P0 was the group of mice without treatment; group P1 was treated with overworking and a recovery period of 48 hours; and group P2 was treated with overworking and a recovery period of 24 hours. After 14 days of treatment, the pancreas of all groups were taken for histological examination using Gomori chrome hematoxylin phloxine B staining. The descriptive analysis showed that the pancreatic tissues of groups P1 and P2 were morphologically damaged compared to group P0. The results of quantitative observations were analyzed by using One way Anova test followed by LSD, indicated that the number of pancreatic β-cells was significantly decreased among the groups (P < 0.05). Conclusion: Acute overtraining may cause damages of pancreatic β cells.Keywords: excessive physical work, pancreatic β cellsAbstrak: Aktivitas fisik berlebihan dapat menyebabkan terjadinya stres oksidatif yang ditandai dengan meningkatnya radikal bebas di dalam tubuh. Stres oksidatif akan menyebabkan kerusakan berbagai macam sel, salah satunya sel β pulau langerhans pankreas. Penelitian ini bertujuan untuk mengetahui aktivitas fisik maksimal akut (acute overtraining) dalam meningkatkan risiko terjadinya diabetes melitus tipe I melalui induksi kerusakan sel β pankreas. Rancangan penelitian ini ialah completely randomized design dengan sampel 36 ekor mencit yang dibagi menjadi 3 kelompok. Kelompok P0 ialah kelompok mencit tanpa perlakuan; kelompok P1 diberi perlakuan beban kerja maksimal dengan periode pemulihan selama 48 jam; dan kelompok P2 diberi perlakuan beban kerja maksimal dengan periode pemulihan selama 24 jam. Setelah 14 hari perlakuan, keseluruhan kelompok sampel diambil organ pankreasnya untuk dibuat sediaan histologik dengan pewarnaan khusus Gomori chrome hematoxylin phloxine B. Analisis deskriptif kualitatif menunjukkan bahwa morfologi jaringan pankreas pada kelompok P1 dan P2 mengalami kerusakan jika dibandingkan P0. Hasil pengamatan kuantitatif dianalisis dengan menggunakan uji One way Anova dilanjutkan dengan Least Significant Difference (LSD), menunjukkan bahwa terjadi penurunan jumlah sel β pankreas yang signifikan antar kelompok P0, P1, dan P2 (P < 0,05). Simpulan: Aktivitas fisik maksimal akut dapat menyebabkan kerusakan sel β pankreas.Kata kunci: beban kerja maksimal, sel β pankreas
Pemberian Ekstrak Daun Cincau (Mesona palustris BL) Meningkatkan Kadar Superoksida Dismutase (SOD) Tikus Wistar (Rattus norvegicus) Jantan yang Diinduksi Latihan Fisik Berlebih Tarnajaya, Karnia; Pangkahila, Alex; Pangkahila, Wimpie; Siswanto, Ferbian M.
Jurnal Biomedik : JBM Vol 10, No 1 (2018): JURNAL BIOMEDIK : JBM
Publisher : UNIVERSITAS SAM RATULANGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35790/jbm.10.1.2018.18995

Abstract

Abstract: This study was aimed to prove that administration of black cincau (Mesona palustris BL) leaf extract increased superoxide dismutase (SOD) levels of overtraining-induced male Wistar rats (Rattus norvegicus). This was a true experimental study using the randomized pretest-posttest control group design. Subjects were 16 male rats (Rattus norvegicus), 6-month old, Wistar strain, weighing 180-200 g, SOD level <39.3 U/mL (normal SOD level), divided into two groups: the control group (P0) and the treatment group (P1). The P0 group was given excessive physical training and aquadest for 14 days, while the P1 group was given excessive physical training and black cincau leaf extract of 54 mg/200 g BW daily for 14 days. The results showed that the mean SOD level before treatment (pretest) in the P0 group was 30.63±2.26 U/mL while in the P1 group was 31.31±2.52 U/mL (P >0.05). After 14-day treatment (posttest), the mean SOD level in the P0 group was 29.36±2.83 U/mL while in the P1 group was 41.31±2.35 U/mL (P <0.01). Further analysis showed that there was no change of SOD levels in the P0 group (P > 0.05), but in the P1 group there was a significant increase of SOD levels from 31.31±2.52U/mL to 41.31±2.35U/mL (P <0.01). Conclusion: Administration of black cincau leaf extract of 54 mg per 200 g of body weight increased SOD level of overtraining-induced male Wistar rats (Rattus norvegicus).Keywords: black cincau leaves, SOD, excessive physical activityAbstrak: Penelitian ini bertujuan untuk membuktikan bahwa pemberian ekstrak daun cincau (Mesona palustris BL) dapat meningkatkan kadar superoksida dismutase (SOD) tikus Wistar (Rattus norvegicus) jantan yang diinduksi pelatihan fisik berlebih. Jenis penelitian ialah eksperimental murni menggunakan randomized pretest-posttest control group design. Subjek penelitian ialah 16 ekor tikus putih jantan (Rattus norvegicus) galur Wistar berumur 6 bulan, sehat, berat badan 180-200 gr, dan kadar SOD <39,3 U/mL (kadar SOD normal), yang dibagi menjadi dua kelompok, yaitu kelompok kontrol (P0) dan kelompok perlakuan (P1), masing-masing berjumlah 8 ekor tikus. Kelompok P0 diberikan pelatihan fisik berlebih dan akuades secara sonde selama 14 hari, dan kelompok P1 diberikan pelatihan fisik berlebih dan ekstrak daun cincau dosis 54 mg/200 gr BB tikus selama 14 hari. Hasil penelitian menunjukkan bahwa rerata kadar SOD sebelum perlakuan (pretest) pada kelompok P0 ialah 30,63±2,26 U/mL, sedangkan pada kelompok P1 ialah 31,31±2,52 U/mL (P >0,05). Setelah perlakuan selama 14 hari (posttest), rerata kadar SOD pada kelompok P0 ialah 29,36±2,83 U/mL, dan pada kelompok P1 ialah 41,31±2,35 U/mL (P <0,01). Hasil analisis efek perlakuan menunjukkan bahwa pada kelompok P0 tidak terjadi perubahan kadar SOD (P >0,05), namun pada kelompok P1 terjadi peningkatan bermakna kadar SOD dari 31,31±2,52 U/mL menjadi 41,31±2,35U/mL (P <0,01). Simpulan: Ekstrak daun cincau dosis 54 mg/200 gr BB tikus dapat meningkatkan kadar SOD tikus Wistar (Rattus norvegicus) jantan yang diinduksi pelatihan fisik berlebih.Kata kunci: daun cincau, SOD, aktivitas fisik berlebih
Co-Authors ., Yulyani ., Zenitalia Boedi P. Yenniastuti, Boedi P. I Made Kardena I Wayan Sugiritama J. A. Pangkahila Linawati, Ni M. Pangkahila, Elionardo Tarnajaya, Karnia Tri A. Putra, Tri A. Wimpie I Pangkahila