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Struktur Anatomi dan Uji Histokimia Terpenoid dan Fenol Dua Varietas Sirih Hijau (Piper betle L.) Gusti Puspa Dewi; Evi Mintowati Kuntorini; Eny Dwi Pujawati
Bioscientiae Vol 17, No 2 (2020): BIOSCIENTIAE VOLUME 17 NO. 2
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (215.764 KB) | DOI: 10.20527/b.v17i2.3448

Abstract

This study aims to determine the anatomical structure and histochemical test of terpenoid and phenol compounds in two varieties of green betel plants (Piper betle). Making leaves anatomical structure preparations using the fresh method, testing terpenoid compounds with 5% copper acetate, testing phenol with ferric trichloride 10% and some grains of sodium carbonate. The observations of the anatomical structure of green betel leaf varieties 1 and varieties 2 have similarities consisting of the upper epidermis, upper hypodermis, palisade parenchyma, parenchymal sponges, vascular bundles (xylem and phloem), sclerenchyma, cholenchyma, lower epidermis, lower hypodermis, secretory cells, trichoma, stoma and calcium oxalate crystals, and in varieties 2 look more trichomes. The anatomical structure of the variety 1 betel stem and varieties 2 are arranged from the outside in the direction of the epidermal tissue, colenchymal tissue, cortical bundles, sclerenchyma, cortex, medullary and peripheral vascular files, pith, the central part of the stem is a secretory gland. Phenol in betel vine varieties 1 and varieties 2 is positive in the secretion cell part which is spread in the parenchymal tissue of the mother's leaf bone and lamina, whereas in the stem is spread around the cortex and pith parenchyma. Positive secretion cells contain phenol not as much as secretory cells containing terpenoids. Based on quantitative observations the size of oil cell density and secretion cell diameter, the essential oils contained in the cell secretions in the leaves of variety 1 are more than varieties 2 while in the varieties 2, there are more varieties 1.
Pengaruh Ekstrak Daun Kepayang (Pangium edule Reinw.) sebagai Bahan Pestisida Nabati pada Mortalitas Ulat Grayak (Spodoptera litura F.) Miaranty archi; Evi Mintowati Kuntorini; Rusmiati Rusmiati
Bioscientiae Vol 17, No 2 (2020): BIOSCIENTIAE VOLUME 17 NO. 2
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (332.092 KB) | DOI: 10.20527/b.v17i2.3449

Abstract

Grayak caterpillar is a pest that always attacks the crops of horticultural and vegetables in Indonesia every growing season that causes farmers to experience crop failure. Kepayang plants contains some secondary metabolite compounds including high glycocyanide acid in each part of the plant. The purpose of this study was to determine the effect and optimal dosage of kepayang leaf extract as a vegetable pesticide on grayak caterpillar mortality. This research used Non-Factorial Randomized Complete Random Design (RCRD) with four treatments in two observation times and each treatment there were three replications. The extraction was done by maceration method using aceton p.a and thickened using rotary evaporator. Mustard as grayak caterpillar feed is dipped in kepayang leaf extract with three dose levels of 1.5gr / L, 1.75gr / L, and 2gr / L is observed for 72 hours and 96 hours. The results showed that pepper leaf extract had an effect on mortality of grayak caterpillar. Variant dose of pepper leaf extract (1.5gr / L, 1.75gr / L, and 2gr / L) in this study have the same cidal effect on grayak caterpillar.