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Christine Makagansa, Christine
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Agriculture, Biological Sciences & Forestry

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KAJIAN AKTIVITAS ANTI-BAKTERI EKSTRAK BIJI PANGI (Pangium edule Reinw) TERHADAP Staphylococcus Aureus, Bacillus Cereus, Pseudomonas Aeruginosa DAN Escherichia Coli SECARA IN VITRO Makagansa, Christine; Mamuaja, Christine F.; Mandey, Lucia C.
Jurnal Ilmu dan Teknologi Pangan Vol 3, No 1 (2015)
Publisher : Pascasarjana Unsrat

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Tujuan utama dari penelitian ini adalah menentukan kandungan fitokimia ekstrak biji pangi dan aktivitas anti-bakteri ekstrak tersebut terhadap pertumbuhan bakteri Staphylococcus aureus, Bacillus cereus, Pseudomonas aeruginosa and Escherichia coli. Ekstraksi menggunakan metode maserasi dengan pelarut akuades. Analisis fitokimia didasarkan pada kandungan total fenol dan tanin terkondensasi. Kandungan total fenolik ekstrak pangi akuades adalah 113,367 mg/kg sedangkan tanin terkondensasi adalah 26,744 mg/kg. Pengujian aktivitas anti-bakteri menggunakan metode difusi cakram kertas. Pada konsentrasi ekstrak 4%, 6% dan 8% efektif menghambat pertumbuhan bakteri Staphylococcus aureus, konsentrasi ekstrak 2%, 4%, 6% dan 8% efektif menghambat pertumbuhan Bacillus cereus. Sedangkan untuk bakteri Escherichia coli dan Pseudomonas aeruginosa, konsentrasi 4%, 6% dan 8% telah menunjukkan efek penghambatan yang efektif, tetapi sedangkan konsentrasi 2% tidak menunjukkan efek penghambatan yang efektif. Kata Kunci: aktivitas anti-bakteri, Bacillus cereus, Escherichia coli, Pangium edule Reinw, Pseudomonas aeruginosa, Staphylococcus aureus
The Antibacterial Activity of Pangi Kernel Extract (Pangium edule Reinw) towards Staphylococcus aureus, Bacillus cereus, Pseudomonas aeruginosa and Escherichia coli in Vitro Makagansa, Christine; Mamuaja, Christine F.; Mandey, Lucia C.
Jurnal Ilmu dan Teknologi Pangan Vol 3, No 1 (2015)
Publisher : Pascasarjana Unsrat

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The aim of this research was to determine the phytochemical content of aquadest extract from pangi kernel (Pangium edule Reinw),and study the antibacterial activity on bacterial growth inhibition of Staphylococcus aureus, Bacillus cereus, Pseudomonas aeruginosa andEscherichia coli. Extraction was carried out done by maceration using aquadest. Analysis of phytochemicals were based on total phenolic and condensed tannins contents. Total phenolic content were 113.367 mg/kg and condensed tannins were 26,744 mg/kg for in 10.000 ppm extract.  Antibacterial activity test was carried out done by using paper disk diffusion method. Extract concentrations of 4%, 6% and, 8% were effectively inhibited Staphylococcus aureus growth,while extract concentration of 2%, 4%, 6% and 8% were efectively inhibited Bacillus cereus respectively. For Escherichia coli and Pseudomonas aeruginosa concentration extract of 2% did not show bacterial inhibition activity, but in concentration extract of 4%, 6% and 8% showed an effective inhibition activity. Keywords : antibacterial activity, Bacillus cereus, Escherichia coli, Pangium edule Reinw,  Pseudomonas aeruginosa, Staphylococcus aureus
Co-Authors Christine Mamuaja Lucia Cecilia Mandey