Dwi Nur Happy Hariyono
Department Of Animal Science, Faculty Of Agriculture, Universitas Khairun, Ternate 97719, Indonesia

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Microsatellite-Based Genetic Diversity Among Three Duck Populations in Sumatera Island Dyah Maharani; Dwi Nur Happy Hariyono; Jafendi Hasoloan Purba Sidadolog; Sunghyun Cho; Prabuddha Manjula; Dongwon Seo; Nuri Choi; Jun Heon Lee
Buletin Peternakan Vol 45, No 2 (2021): BULETIN PETERNAKAN VOL. 45 (2) MAY 2021
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v45i2.37816

Abstract

This study aimed to determine the genetic diversity among three duck populations (Bayang, Pegagan, and Pitalah) reared in Sumatera island, Indonesia, using microsatellite markers. Genetic diversity among populations (n = 90) was determined using 22 microsatellite markers, based on several indices: number of alleles (Na), observed heterozygosity (Ho), expected heterozygosity (He), polymorphism information content (PIC), and Wright’s F-statistics ( ). The total number of alleles detected across loci was 121. The Na per locus ranged from 2 (APH24, CAUD128, and CAUD009) to 18 (CAUD048 and CAUD040). The mean Ho (0.429) dan He (0.509) indicated that the level of genetic diversity among populations was moderate, while the mean PIC (0.46) suggested that the tested loci were informative for assessing genetic diversity. The mean F-statistics ( ) were 0.148, 0.198, and 0.060, respectively. The  value indicated that the level of genetic differentiation among populations was moderate. The results confirms a moderate genetic diversity among populations, which could be beneficial for designing conservation and utilization of the local ducks in Sumatera island.
IDENTIFIKASI KARAKTERISTIK FISIK TERNAK KAMBING DI PULAU TIDORE Eny Endrawati; Sri Lestari; Dwi Nur Happy Hariyono
Agrivet : Jurnal Ilmu-Ilmu Pertanian dan Peternakan (Journal of Agricultural Sciences and Veteriner) Vol. 10 No. 1 (2022): Juli
Publisher : Fakultas Pertanian Universitas Majalengka

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (354.57 KB) | DOI: 10.31949/agrivet.v10i1.2731

Abstract

The aim of this study was to identify the potential of goat farming on Tidore island through the identification of physical characteristics, e.g. body coat color, coat color pattern, facial profile, beard profile, and back profile. The physical characteristics of 101 goats (11 males and 90 females; aged 1 to 2 years) were observed, tabulated, and analyzed using descriptive statistics as number of observations and percentage. The results showed that the majority of the studied goats had the following physical characteristics: a combination of brown and white and of black, white, and brown for body coat color in males (45.45%) and females (27.78%), respectively, striped coat color pattern (63.64% in males; 78.89% in females), straight facial profile (81.82% in males; 95.56% in females), bearded in males (63.64%) and beardless in females (95.56%), and straight back profile (81.82% in males; 91.11% in females). It can be concluded that the studied goat population had variations in the physical characteristics, suggesting their potential use as a basis for development of goat genetic resources on Tidore island.
Application of Microsatellite Markers for Genetic Diversity Analysis of Indonesian Local Cattle Dwi Hariyono
WARTAZOA. Indonesian Bulletin of Animal and Veterinary Sciences Vol 32, No 2 (2022): June 2022
Publisher : Indonesian Center for Animal Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/wartazoa.v32i2.3040

Abstract

Animal genetic resources (AnGR), including cattle, have been valuable national assets that need to be preserved and developed. There are at least 16 recognized breeds of cattle that have been registered as local and new breeds by the Ministry of Agriculture of the Republic of Indonesia. Conservation and development programs of these local cattle breeds require basic information regarding their genetic diversity, relationships, and structures. There are several types of DNA markers that can be used for genetic diversity analysis, such as microsatellite markers. Microsatellites or short tandem repeats (STRs) are a group of DNA sequences consisting of tandemly repeated units (1–6 bp), which are abundant throughout the genome and can be found in both coding and non-coding regions. The primary advantages of microsatellites are that they are inherited in a Mendelian pattern (codominant markers), high polymorphism rates, and high abundances throughout the genome. The aim of this review is to discuss the application of microsatellite markers for genetic diversity analysis in Indonesian local cattle based on 3 indices: number alleles per locus, expected heterozygosity (He), and polymorphisms information content (PIC). There are at least 28 microsatellite markers that have been studied in Indonesian local cattle, with the number of alleles per locus ranging from 2 to 32, He values ranging from 0.100 to 0.985, and PIC values from 0.095 to 0.935. Based on the PIC values, several microsatellites are classified as highly informative, e.g. BM1824, ILST6, TGLA126, TGLA53, TGLA227, TGLA122, ETH225, INRA23, SPS113, SPS115, BM1818, CSSM66, ETH10, INRA005, INRA037, ETH185, HEL017, and ILSTS029. Therefore, these microsatellite markers can be potentially used for future genetic diversity analysis of other breeds of cattle.
Body condition score sapi Bali induk dan temperature-humidity index di Kecamatan Tidore Utara, Kota Tidore Kepulauan Maman Jafar; Eny Endrawati; Dwi Nur Happy Hariyono
Agrivet : Jurnal Ilmu-Ilmu Pertanian dan Peternakan (Journal of Agricultural Sciences and Veteriner) Vol. 11 No. 1 (2023): Juni
Publisher : Fakultas Pertanian Universitas Majalengka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31949/agrivet.v11i1.4771

Abstract

This study aimed to determine the body condition score (BCS) of Bali cows kept on smallholder farms and the temperature-humidity index (THI) in the Tidore Utara sub-district, from June to July 2022. Eighteen cows (2-3 years old) were selected on the basis of their BCS through direct observation (scoring scale: 1-5). The BCS data were analyzed as percentages (%) for each scoring scale and presented in a chart. The THI value was determined based on temperature (ºC) and humidity (%) data and analyzed using descriptive statistics as mean and standard deviation. The results showed that the average temperature in the morning and evening at the study site was relatively the same (32.00±3.14ºC vs. 32.01±1.17ºC), while the average humidity in the two-time groups was relatively different (87.89±11.14% vs. 77.96±6.50%). The average THI in the morning and evening (88.71±3.23 vs. 85.67±1.00) indicated that the cows in the study site experienced severe heat stress. The majority of the cows had BCS 3 (46.67%), while the remaining cows had BCS 2 (33.33%), BCS 4 (13.33%), and BCS 1 (6.67%). None of the cows had BCS 5 (0%).