Article Per Year (5 Year)
p-Index From 2021 - 2026
0.23
P-Index
This Author published in this journals
All Journal HAYATI Journal of Biosciences Makara Journal of Health Research
Abdullah, Kahairi
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences

Published : 3 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 3 Documents
Search

 1 
Quality of Life in Pre- and Post-Treatment among Head and Neck Cancer Survivors at A Tertiary Centre, Malaysia Ludin, Salizar M; Abdullah, Che-Azunie C; Abdullah, Kahairi
Makara Journal of Health Research Vol. 22, No. 2
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Background: Treatment for head and neck cancer (HNC) may result in a variety of long term consequences that impair their health and quality of life (QoL). HNC patients often are prone to have a poor health related QoL due to significant changes in vital functions. Despite researches being done in the area cancer survivors' QoL internationally, those done locally were relatively scares and not clear. The study aim to determine and compares the pre and post treatments QoL in HNC patients. Methods: A cohort study was carried out to recruit 81 newly diagnosed HNC patients purposively; pre and six months post treatment using QoL Cancer Survivor and Questionnaire-Head &Neck 35 questionnaires. .Data was analyzed for descriptive and inferential analysis. Results: A total of 40 (50%) patients participated and the result shows that QoL of HNC patients were at medium level (6.22 ± 1.8) pre-treatment, and however reduced (4.84 ± 1.16) at 6 months post treatment), despite the symptoms or problems that occur reduced post treatment compared (1.58 ± 1.25). The Pearson coefficient correlation test result shows that QoL of HNC patients pre and post treatment were strong but negatively correlated (r = -0.447, p = 0.002). Conclusion: Understanding of QoL and affecting factors in HNC patients' QoL is very crucial as it may potentially assist in designing interventions that lessen the adverse impact of this disease process and more accurately support those in active treatment, survivors, and caregivers.
Detectability of circulating microRNAs in microRNA extracts with low purity and yield using quantitative real-time polymerase chain reaction: Supporting evidence Ahmad, Azmir; Kaderi, Mohd. Arifin; Tumian, Afidalina; Sivanesan, Vijaya Mohan; Abdullah, Kahairi; Leman, Wan Ishlah; Mohamad, Irfan; Wan Zainon, Wan Mohd. Nazri; Mohd. Shiyuti, Muhammad Izani; Mohamed Awang, Kamariah; Rosla, Luqman; Paul, Mark; Syed Yussof, Sharifah Nor Ezura; Ramli, Rosdi
Makara Journal of Health Research Vol. 24, No. 3
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Background: Circulating microRNAs (miRNAs) are a group of noncoding RNAs with promising potential as minimal invasive biomarkers for noncommunicable diseases. However, challenges exist in the preparation of these miRNAs from peripheral blood samples for quantification purposes. The low quality of miRNA extracts presents an obstacle. Acknowledging the superior performance of quantitative real-time polymerase chain reaction (qPCR) as gold standard for gene expression analysis, we conducted this study to observe the capabilities of qPCR using the Taqman® protocol in amplifying circulating miRNAs from miRNA extracts with low purity and yield. Methods: miRNAs were extracted from thirty-six plasma samples that were obtained from public subjects. Four selected miRNAs were quantified using the Taqman® protocol in an integrated fluidic circuit chip that was optimized from a previous study. The amplification graph and Cq values were obtained to observe any abnormal amplification signs and expression levels, respectively. Results: The qualitative observation of the amplification of the four miRNAs showed no sign of abnormality, thereby indicating the successful amplification of the miRNAs without enzymatic inhibition. Furthermore, the miRNAs were quantified in high expression levels. Conclusion: The circulating miRNA extracts with low purity and yield were practical for the study of circulating miRNA expression based on the Taqman® protocol as the method of detection.
Quantile Normalization for High Throughput Circulating MicroRNA Expression Study using TaqMan® Low Density Array Panels: Supporting Evidence Ahmad, Azmir; Mohamed, Syarah Syamimi; Tumian, Afidalina; Tolos, Siti Marponga; Sivanesan, Vijaya Mohan; Leman, Wan Ishlah; Abdullah, Kahairi; Mohamad, Irfan; Wan Zainon, Wan Mohd. Nazri; Rosla, Luqman; Syed Yussof, Sharifah Nor Ezura; Mark, Paul; Mohamed@Awang, Kamariah; Ramli, Rosdi; Omar, Eshamsol Kamar; Mohd. Yassin, Mohd. Wardah; Mohamad, Mohd. Amin Marwan; Kaderi, Mohd. Arifin
HAYATI Journal of Biosciences Vol. 31 No. 3 (2024): May 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.3.432-442

Abstract

In searching for new biomarkers, high throughput technique has been widely used by researchers, including for gene expression study. However, the reliability and accuracy of results from high throughput study critically depends on appropriate data management, including normalization methods. Data driven normalization has been introduced as a normalization method for high throughput gene expression study. Thus, this study was conducted to evaluate the performance of various data driven and reference genes normalization methods using a high throughput circulating microRNA expression dataset. A quantification cycle (Cq) dataset generated from a high throughput circulating microRNA study was used to test the normalization methods using HTqPCR package in R software. The normalized Cq generated from different methods were compared descriptively using box plot analysis and coefficient of variance. The box plot analysis showed that quantile normalization produced more homogenous Cq distribution, lesser outliers and reduced coefficient of variance as compared to other normalization methods in screening and validation phases. The overview on quantile normalized Cq showed consistency in its level of expression before and after 2-∆∆Cq calculation indicating the reliability of quantile normalized Cq. Quantile normalization is suggested to be used in high throughput miRNA expression study due to its performance in homogenizing the data, reduce outliers and coefficient of variance.
Co-Authors Abdullah, Che-Azunie C Ahmad, Azmir Kaderi, Mohd. Arifin Leman, Wan Ishlah Ludin, Salizar M Mark, Paul Mohamad, Irfan Mohamad, Mohd. Amin Marwan Mohamed Awang, Kamariah Mohamed, Syarah Syamimi Mohamed@Awang, Kamariah Mohd. Shiyuti, Muhammad Izani Mohd. Yassin, Mohd. Wardah Omar, Eshamsol Kamar Paul, Mark Ramli, Rosdi Rosla, Luqman Sivanesan, Vijaya Mohan Syed Yussof, Sharifah Nor Ezura Tolos, Siti Marponga Tumian, Afidalina Wan Zainon, Wan Mohd. Nazri