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Characterization of Cellulase Enzyme Produced by Two Selected Strains of Streptomyces Macrosporeus Isolated from Soil in Indonesia Soeka, Yati; Suharna, Nandang; Triana, Evi; Yulinery, Titin
Makara Journal of Science Vol. 23, No. 2
Publisher : UI Scholars Hub

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Abstract

This study was conducted to characterize the cellulase enzymes produced by Streptomyces macrosporeus BB 32 and S.microspores KRC 21. D, which were isolated from Tanjung Pendam, Belitung Island, and from Cibodas Botanical Gar-den, Indonesia, respectively. The optimal activity of the enzymes was analyzed using parameters such as incubationtime, pH, temperature, carboxymethylcellulose (CMC) concentration. The effect of the addition of some metal ions asactivators or inhibitors was also analyzed spectrophotometrically at λ 540 nm. Results demonstrated that the activity of the cellulase enzymes of S. macrosporeus BB 32 and S. macrosporeus KRC 21 D reached the optimum level after 2 and5 days of incubation and at pH values of 8.0 and 6.0, temperatures of 35 °C and 50 °C, and CMC concentrations of 1.75% and 2%, respectively. S. macrosporeus BB 32 cellulase was activated by the cations CuCl2, MgCl2, and ZnCl2but inhibited by NaCl and CoCl2, reducing its activity. The cellulase of S. macrosporeusKRC 21.D was activated bythe cation NaCl and by the divalent cations CoCl2, CuCl2, MgCl2, and ZnCl2.S. macrosporeus BB 32 was deposited at the Indonesian Culture Collection with the collection number InaCC A144.
Studi Penerapan Cara Produksi Pangan Yang Baik (CPPB) dan Umur Simpan Mi Glosor Di Kota Bogor Soeka, Yati; Jumiono, Aji
Jurnal Ilmiah Pangan Halal Vol. 1 No. 1 (2019): Jurnal Ilmiah Pangan Halal
Publisher : Universitas Djuanda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30997/jiph.v1i1.2006

Abstract

One of the fast food sold by  small and medium businesses is noodles. Noodles are preferred by consumers because they are cheap, affordable for all levels of society, can be processed  into a variety of cuisines, and can be served quickly. One of the most common culinary noodles consumed by the people of Bogor city and its surroundings is the glutinous noodles which are widely served with various variations. However, in buying, sometimes people do not know whether the glossy noodles they consume are suitable foods to consume. This research aims to know  the making of glossy noodles by the glossy noodles maker in Bogor city, based on good food production  methods so that we can  know the shelf life of glossy noodles in the laboratory by analyzing the total plate count and organoleptic test  with packaging technique of HDPE plastic bags stored  for 5 days, heat-resistant HDPE plastic sealed stored for 10 days and heat-resistant HDPE plastic which was vacuum stored for 15 days with no dyeing and with immersion treatments in water of 100 oC for 1 minute. Data on good food production methods is obtained by means of surveys that reveal  case studies of a problem with a qualitative approach. The results of research showed that the results of observation of small and medium business in CV Taruna  have four major activities, namely procurement of materials, production process, packaging, and marketing or delivering. Small and medium businesses of  CV Taruna have 37 process flows. The conditions of implementing good food production methods  are currently at level 4. The number of serious  nonconformities of 3 elements and critical  mismatches of 2 elements. The results of the analysis of the total plate numbers with packaging technique of HDPE plastic bags stored  for 5 days, heat-resistant HDPE plastic sealed stored for 10 days and heat-resistant HDPE plastic which was vacuum stored for 15 days with no dyeing treatment and with immersion in water of 100 oC for 1 minute meet SNI requirements < 1x10-6 at room temperature for 72 hours. The results of organoleptic test with packaging technique of HDPE plastic bags stored  for 5 days, heat-resistant HDPE plastic sealed stored for 10 days and heat-resistant HDPE plastic which was vacuum stored for 15 days with no dyeing treatment and with immersion in water of 100 oC for 1 minute for color, aroma, texture, and appearance on the score from dislike to very  like. Keywords: small and medium enterprises, gloss noodles, shelf life, total plate count, organoleptic test.