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All Journal Journal of the Indonesian Medical Association : Majalah Kedokteran Indonesia Makara Journal of Science InaBHS (Indonesian Journal of Biomedicine and Health Science)
Pratiwi, Ekawati Betty
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Biochemistry, Genetics & Molecular Biology Education Health Professions Medicine & Pharmacology Public Health

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Computational Analysis of Chromophore Tripeptides FollowingFusion of Enhanced Green Fluorescent Protein and Cell-penetrating Peptides Widyaningtyas, Silvia Tri; Pratiwi, Ekawati Betty; Bela, Budiman
Makara Journal of Science Vol. 24, No. 4
Publisher : UI Scholars Hub

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Abstract

Cell-penetrating peptides (CPPs) are small peptides that can transfer other materials into a cellular compartment. In this research, we studied the effect of fusion of new CPPs to the N-terminal of enhanced Green Fluorescent Protein eGFP on the ability of the latter to fluoresce. Results showed that the recombinant protein CPPs-eGFP could be successfully expressed in Escherichia coli. In contrast to E. coli expressing wild-type eGFP, which could fluoresce under ultraviolet (UV) or visible light, E. coli expressing CPPs-eGFP lost their ability to fluoresce. PyMol, a molecular visualization system, revealed that fusion of the new CPPs to the N-terminal of eGFP alters interactions between chromophore-forming tripeptides and the adjacent amino acids of other tripeptides. Disrupting peptide interactions induced structural changes in eGFP that caused it to lose its fluorescence ability. We suggest performing computational analyses to predict the biological function of new fusion proteins prior to starting laboratory work.
Mengatasi Tantangan Skrining HPV pada Perempuan: Sebuah Kajian Umum Bela, Budiman; Hartono, Soni; Pratiwi, Ekawati Betty; Cahyani, Hesti Pramesti; Widyaningtyas, Silvia Tri
Majalah Kedokteran Indonesia Vol 75 No 2 (2025): Journal of The Indonesian Medical Association - Majalah Kedokteran Indonesia, Vo
Publisher : PENGURUS BESAR IKATAN DOKTER INDONESIA (PB IDI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47830/jinma-vol.75.2-2025-1898

Abstract

Introduction: A major challenge in the success of HPV screening programs is achieving 70% coverage of women aged 30-69. This study conducted a survey to examine the factors influencing the willingness of woman in that age group to actively engage HPV screening program.Method: Socio-demographic data, knowledge levels, cervical cancer screening uptake, and willingness to participate in the government’s routine screening program were collected digitally using cross-sectional study. Factors associated with knowledge, participation, and willingness to engage program measured using logistic regression analysis.Result: Among the 87 eligible respondents, 96.6% had heard about cervical cancer mostly via internet, and 51% categorized as having low level knowledge. The percentage of participants willingness to participate in program was 67.82% below the percentage government target coverage. Factors correlated with willingness included: age group 36-40 (aOR = 1.865, 95% CI = 0.567-6.129) and 41-45 (aOR = 2.93, 95% CI = 0.762-11.272), monthly household expense (aOR = 1.682, 95% CI = 0.684-4.138), having relatives suffer cervical cancer (aOR = 2.764, 95% CI = 0.790-9.672), history of HPV vaccination (aOR = 1.187, 95% CI = 0.593-7.539).Conclusion: Respondent willingness to participate in HPV screening program remains to be improved. Various ways to increase participation in screening programs need to be developed to reach various populations of women.
Pemilihan Peptida Immunogenik E6 Human Papiloma Virus 16 sebagai Kandidat Vaksin kuratif dengan menggunakan Next-Generation IEDB Tools Widyaningtyas, Silvia Tri; Pratiwi, Ekawati Betty; Bela, Budiman
InaBHS (Indonesian Journal of Biomedicine and Health Science) Vol 3 No 2 (2024): Indonesian Journal of Biomedicine and Health Science
Publisher : Fakultas Kedokteran UGJ Cirebon

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33603/inabhs.v3i2.10519

Abstract

ABSTRAK Kanker serviks atau kanker mulut rahim merupakan salah satu penyebab kematian tinggi sampai saat ini. Kanker ini dikaitkan dengan infeksi Human Pappiloma virus (HPV). Vaksinasi merupakan salah satu metode pencegahan yang cukup efektif untuk mencegah kanker serviks. Vaksin yang beredar saat ini efektif untuk mencegah infeksi HPV, namun kurang efektif jika diberikan pada orang yang telah terinfeksi. Hal tersebut dikaitkan dengan jalur kekebalan tubuh yang diinduksi oleh vaksin tersebut. Dalam penelitian ini dirancang antigen HPV 16 yang dapat digunakan dalam pengembangan vaksin terapetik yang ditujukan untuk meningkatkan respon kekebalan seluler yand secara spesifik dapat menghancurkan sel kanker servik. Pemilihan dan karakterisasi antigen dilakukan dengan menerapkan Bioinformatika. Parameter yang dinilai antara lain kemampuan antigen diproses dan dipresentasikan oleh sel tubuh pada sel kekebalan tubuh yang bertanggung jawab pada eliminasi sel kanker, yaitu sel CD8. Hasil studi menunjukkan diperoleh epitop CD8 yang dikenali oleh beberapa jenis HLA. Bioinformatika dapat digunakan untuk mencari epitop.
Co-Authors Budiman Bela Cahyani, Hesti Pramesti Hartono, Soni Widyaningtyas, Silvia Tri