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All Journal Jurnal Periodic Jurusan Kimia UNP
Nailul Rahmi
Jurusan Kimia FMIPA, Universits Negeri Padang, Padang, Indonesia
Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Energy Materials Science & Nanotechnology

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Penentuan Kadar Etanol Pada Sampel Minuman dengan Metoda HPLC Menggunakan Fasa Gerak Asetonitril dan Buffer Fosfat Nailul Rahmi; Budhi Oktavia; Nazulis Z
Jurnal Periodic Jurusan Kimia UNP Vol 2, No 1 (2013)
Publisher : Departemen Kimia FMIPA UNP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (268.642 KB) | DOI: 10.24036/p.v2i1.2373

Abstract

Alcoholic beverage is a drink containing ethanol. Ethanol is a psychoactive substance and its consumption causes loss of consciousness. All types of alcohol are essentially toxic, especially if consumed in excess, such as ethanol. According to  BPOM limit of the use of alcohol in drinks are ± 1% - 5%. Ethanol content analysis in this study using HPLC method with condition UV-Vis using a mobile phase of acetonitrile: phosphate buffer (5: 95) pH 6 at 220nm wavelength detector  and the use of stationary phase Zorbax Rx C18 ODS column. The result of this method  is retention time for ethanol 1.68 minutes. The aplication of the method for determination of ethanol on the drinks from the market for 5 different samples was tested. The sample consists of 3 types of alcoholic beverage production factory/industrial, and 2 types of alcoholic beverages produced household. With adition standard techniques on samples was obtained ethanol adduct levels in 2 samples are 20.416% and 3.80%
Co-Authors Budhi Oktavia Nazulis Z