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Wiwik Hardaningsih
Universitas Tamansiswa Padang
Agriculture, Biological Sciences & Forestry Immunology & microbiology Veterinary

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Kryopreservasi Sebagai Upaya Konservasi Plasma Nutfah Jangka Panjang Secara in Vitro Beberapa Genotipe Pisang (Musa Spp L.) Wiwik Hardaningsih
Jurnal Embrio Vol 5 No 02 (2012): Jurnal embrio
Publisher : Fakultas Pertanian Universitas Tamansiswa Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (149.122 KB) | DOI: 10.31317/embrio.v5i02.132

Abstract

Research on long-term conservation of germplasm various banana genotypes (Musa spp L.) with kryopreservasi in liquid nitrogen (-196oC temperature) has been conducted at the Laboratory of Tissue Culture and Germplasm Conservation ITFRI Solok. This study aims to: know the method of conservation of banana germplasm with long-term conservation kryopreservasi suitable for a variety of banana genotypes. The results showed that: kryopreservasi method that can used for long term storage is a method of slow freezing (slow cooling cryopreservation) by using the vitrification method given the addition of PVS2 cryoprotectant atthawing temperatures 40oC.
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