Garuda - Garba Rujukan Digital

Aryadi Subrata

Unknown Affiliation

Author-ID : 5420916

Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Immunology & microbiology Medicine & Pharmacology

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Efek Antibakteri Ekstrak Etanol Daun Pandan Wangi (Pandanus Amarylifolius) Terhadap Enterococcus Faecalis (In Vitro) Aryadi Subrata; Valencia Lawrence
Jurnal Kedokteran Gigi Terpadu Vol. 3 No. 2 (2021): Jurnal Kedokteran Gigi Terpadu
Publisher : Fakultas Kedokteran Gigi Universitas Trisakti

Background: Enterococcus faecalis is a bacterium that is commonly found in pulp necrosis and is difficult to get rid of, causing endodontic persistence. Irrigation materials which are often used these days still have some limitations that can lead to failure of root canal treatment. Pandanus amarylifolius leaf is an herbal ingredient that has several active compounds such as flavonoids, polyphenols, saponins, and alkaloids are known for having antibacterial effects which are expected to inhibit E. faecalis . Objective: To determine the antibacterial effect of ethanol extract of pandan wangi leaves on the growth of bacteria E. faecalis. Method: Experimental laboratory with well diffusion method. The ethanol extract of wangi pandan leaves was obtained by maceration method using 96% ethanol as a solvent. Aquades was used as a negative control, 0,2% chlorhexidine as a positive control, and the concentrations of ethanol extract of Pandan wangi leaves used were 6.25%, 12.5%, 25%, 50%, and 100%. Results: The ethanol extract of pandan wangi leaves had an antibacterial effect on the growth of E. faecalis. Extract concentrations of 6.25%, 12.5%, and 25%, don’t have inhibiton zone. Extract concentrations 50% and 100% had an average inhibition zone diameter of 11,400 ± 2,621 mm; The positive control shows the average inhibition zone of 16,094 ± 2,981 mm, while the negative control does not have an inhibition zone. Conclusion: Ethanol extract of pandan wangi leaves has an antibacterial effectivity against E. faecalis with the concentration 50% and 100%.

Efek Antibakteri Ekstrak Daun Mangrove (Acanthus Ilicifolius) Dengan Tiga Jenis Pelarut Terhadap Bakteri Enterococcus Faecalis(In Vitro) Aryadi Subrata; Melisa Putri Usboko
Jurnal Kedokteran Gigi Terpadu Vol. 3 No. 2 (2021): Jurnal Kedokteran Gigi Terpadu
Publisher : Fakultas Kedokteran Gigi Universitas Trisakti

Background: Irrigation is a stage to completely clean root canals when RCT is carried out to prevent recurrent infections. Increased antibiotic resistance and the side effects of using chemical irrigation solutions have led to the search for alternative herbal ingredients. Mangrove leaves (Acanthus ilicifolius) are known to contain bioactive compounds that can be effective antibacterial agents against gram-positive bacteria. Objective: To determine the differences in the antibacterial effect of Acanthus ilicifolius mangrove leaf extract with methanol, ethyl acetate and aquades solvents against Enterococcus faecalis bacteria. Methods: The leaves of Acanthus ilicifolius were extracted with maceration method using methanol (polar), ethyl acetate (semi-polar) and aquades (non-polar) and then diluted in concentrations of 1.56%, 3.13%, 6.25%, 12,5%, and 25%, chlorhexidine 2% as positive control and aquades as negative control. The extract effectiveness test was carried out with dilution method by mixing bacteria and extract each 20µl into 96 well-plate wells then incubated for 24 hours, followed by the diffusion method by inoculating as much as 5µl of the dilution results on a plate containing agar medium, then incubated for 24 hours. The number of bacterial colonies was then counted manually. Results: The extract had an antibacterial effect against Enterococcus faecalis, seen from a decrease in the number of bacterial colonies compared to negative controls, but not better than positive controls. The extract with the lowest number of colonies was found with a 25% concentration of methanol solvent. Data analysis was performed using the Kruskal Wallis test. Conclusion: There is no significant difference in antibacterial effect between the extract with the three different solvents. The extract with a concentration of 25% methanol as a solvent could not replace 2% chlorhexidine.

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