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All Journal International Journal of Advances in Applied Sciences Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences
Ida Bagus Wayan Gunam
Udayana University
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences Environmental Science Materials Science & Nanotechnology Mathematics Physics

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The Selection and the Growth Condition Optimization of Ethanol‐Producing Microbes Isolated from Ragi Tapai: Selection and the Growth Condition Optimization of Ethanol‐Producing Microbes Ida Bagus Wayan Gunam; I Gede Arya Sujana; I Made Mahaputra Wijaya; Yohanes Setiyo; I Wayan Arnata; I Wayan Wisma Pradnyana Putra
Journal of Tropical Life Science Vol. 13 No. 2 (2023)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.13.02.12

Abstract

Ethanol is a biofuel produced from renewable resources, which potentially plays an important role in solving future fuel problems. This study aimed to select the highest ethanol-producing isolate from candidates obtained from previously isolated candidates from ragi and cassava tapai. The selection process was conducted in 2 stages, namely: 1) Selection of the highest ethanol-producing isolate from seven isolate candidates using PYG media containing peptone, yeast extract, and glucose at 0.75%, 0.75%, and 15%, respectively and was followed by: 2) Optimization of the growth conditions of the highest ethanol-producing isolate, which was conducted at various temperatures of 27, 30, 33, 35, 37, and 40°C with the combination of various pH of 3.5, 4.5, 5.5, and 6.5. The experimental results showed that the R5I3 isolate was the highest ethanol-producing performance isolate, which yielded approximately 4.69±0.25% (v/v). Following the temperature and pH optimization of the fermentation processes, the optimum growth conditions were at 35°C and pH 5.5, where the ethanol produced was increased to 8.63 ± 0.04% (v/v). With these results, this new strain has the potential to be used in bioethanol production processes and other industrial applications.
Temperature and pH effects on bioethanol production from wild cassava (Manihot glaziovii Muell. Arg) using simultaneous co-fermentation Ida Ayu Pridari Tantri; Ida Bagus Wayan Gunam; Anak Agung Made Dewi Anggreni; I Gede Arya Sujana
International Journal of Advances in Applied Sciences Vol 15, No 1: March 2026
Publisher : Institute of Advanced Engineering and Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11591/ijaas.v15.i1.pp226-234

Abstract

Bioethanol is a clean alternative energy source, with wild cassava (Manihot glaziovii Muell. Arg) as a potential feedstock. Fermentation converts glucose from hydrolysis into ethanol. This study examines the effects of pH and fermentation temperature on bioethanol characteristics using a simultaneous saccharification and co-fermentation (SSCF) technique. A factorial randomized block design (RBD) was used with two factors: pH (4.5, 5.0, and 5.5) and fermentation temperature (30, 32.5, and 35 °C). Data were analyzed using variance and Duncan’s test. Results showed that pH and temperature significantly affected pH value, total soluble solids, reducing sugar, and ethanol content. The optimal conditions for bioethanol production were pH 4.5 and temperature 32.5 °C, yielding a pH of 3.55±0.07, total soluble solids of 9.3±0.57 °Brix, reducing sugar of 3.038±0.10 mg/mL, and ethanol content of 3.48±0.37 (%w/v). Based on the results of this study, wild cassava can be utilized as bioethanol by considering the effect of fermentation conditions.
Co-Authors Anak Agung Made Dewi Anggreni I Gede Arya Sujana I Gede Arya Sujana I Made Mahaputra Wijaya I Wayan Arnata I Wayan Wisma Pradnyana Putra Ida Ayu Pridari Tantri Yohanes Setiyo