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All Journal Jaringan Laboratorium Medis
Sabrina Dela Agiska
Jurusan Analis Kesehatan Prodi Teknologi Laboratorium Medis
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Public Health

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Validasi Pemeriksaan Kolesterol Total Metode CHOD- PAP pada Sampel yang Disimpan Selama 1 Minggu dalam Suhu 4-8°C Jihan Nabilla; Nabila Intifa Ulya; Rieke Rofikoh Ardianty; Sabrina Dela Agiska; Fatkhatin Nadifah; Nurul Qomariyah
Jaringan Laboratorium Medis Vol 5, No 1 (2023): May 2023
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v5i1.8690

Abstract

High total cholesterol levels cause various diseases, especially those related to the heart  and blood vessels. Total cholesterol test uses serum as a sample and must be analyzed within one hour after sampling.However, in the field sometimes there are inspection delays.Some studies have proven that serum stored for one week at a temperature of 4-8°C has different results in total cholesterol examination. For this reason, this study aims to determine the validity of total cholesterol examination using the CHOD-PAP method of serum samples stored for one week at a temperature of 4o-8oC. Validation test based on eight parameters, there are precision, accuracy, linearity, reportable range,limit detection, quantitation limit,  recovery, and interference. Only the recovery test was not accepted from eight parameters. Based on the Kruskal Wallis test, the sig value is 0.172 0.05, it can be stated that there is no difference in serum storage time for one week at a temperature of 4-8℃ on total cholesterol levels using CHOD-PAP method.
Co-Authors Fatkhatin Nadifah Jihan Nabilla Nabila Intifa Ulya Nurul Qomariyah Rieke Rofikoh Ardianty