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Evelina Somar
Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Papua
Agriculture, Biological Sciences & Forestry Chemistry Mathematics Physics

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ISOLASI, IDENTIFIKASI DAN UJI AKTIVITAS ANTIBAKTERI MINYAK ATSIRI DARI KULIT KAYU AKWAY (Drimys arfakensis Gibbs) ASAL PEGUNUNGAN ARFAK PAPUA Hajjah Lita Chilviana Hajjah Lita Chilviana; Bimo Budi Santoso; Evelina Somar
Jurnal Natural Vol. 18 No. 2 (2022): Jurnal Natural
Publisher : FMIPA Universitas Papua

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30862/jn.v18i2.196

Abstract

ABSTRACT Isolation of essential oil by steam and water distillation method, identification by GC-MS and antibacterial activity test against essential oil of akway bark (D. arfakensis Gibbs) have been carried out. The results showed that the essential oil component of akway bark was composed of 142 compounds, which were dominated by 10 components. The ten suspected components are: 4,5-dimethyl-1-heksen (4,15%); 4-methyl-1-ol-3-penten (3,93%); 1,4-benzodioksan-2,3-diyldiacetate (3,59%); (E) 2,6-dimethyl-1,6-diol-2,7-octadene (3,32%); 1[5(1-Hidroxyethylidene)-1,3-cyclopentadien-1-yl]-ethanone; (3,28); 2-(2,5-hexadiynyloxy)tetrahydro-2H-pyran (3,08%); 3,7-dimethyl-1,6-octadiene (3,03%); 2-methyl-2-ol-4-penten (2,52%); 3-methyl-1-ol-4-penten (2,51%); 4,8-dimethyl-4-ol-1,7-nonadien (1,85%). Testing the antibacterial activity of Akway bark (D. arfakensis Gibbs) using the well diffusion method against four test bacteria, namely B. subtilis, E. coli, S. aureus, and S. typhi, showed that Akway essential oil (D. arfakensis Gibbs) had Inhibition with strong to very strong category. The inhibition of essential oils against the test bacteria B. subtilis, E. coli, S. aureus, and S. typhi, each had inhibition zones of 25 mm, 16.5 mm, 34 mm, and 22 mm. Keywords: D. arfakensis Gibbs., B. Subtilis, E. Coli, S. aureus, S. typhi and essential oil composition.
SKRINING FITOKIMIA DAN UJI TOKSISITAS EKSTRAK DAUN BUAH HITAM (Haplolobus cf. Monticola Husson) DENGAN METODE BRINE SHRIMP LETHALITY TEST (BSLT) AIsyah Ayu Indarsari; Evelina Somar; Sabir Sumarna
Jurnal Natural Vol. 19 No. 1 (2023): Jurnal Natural
Publisher : FMIPA Universitas Papua

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30862/jn.v19i1.176

Abstract

Indonesia is a country with the second highest terrestrial biodiversity wealth in the world. Some areas have endemic types of flora and fauna. One of the endemic flora found in Papua and widely found in Teluk Wondama district is piarawi or black fruit (Haplolobus cf. monticola Husson). The results showed that black fruit plants, both fruit and leaves, contain secondary metabolites that can cause pharmacological effects. The effectiveness of these active components as herbal medicines can be determined through a preliminary analysis in the form of a toxicity analysis. So far there has been no research on the content of secondary metabolites in the methanol extract of black fruit leaves. Thus, in this study, phytochemical and toxicity tests were carried out on the methanol extract of black fruit leaves (Haplolobus cf. monticola Husson) using the Brine Shrimp Lethality Test (BSLT) method. This test provides an overview of the toxicity level of black fruit leaf extract on Artemia salina Leach shrimp larvae. The test results can be used to identify bioative metabolites contained in black fruit leaves. Based on the results of the study, it can be concluded that the methanolic extract of black fruit leaves contains secondary metabolites such as alkaloids, flavonoids, saponins and tannins. Meanwhile, the results of the toxicity test showed that the LC50 value of the methanol extract of black fruit leaves was 102.5473779 ppm 102.53, so it could be categorized as moderately toxic.
Co-Authors AIsyah Ayu Indarsari Bimo Budi Santoso Hajjah Lita Chilviana Hajjah Lita Chilviana Sabir Sumarna