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All Journal Asian Journal of Aquatic Sciences
Iesje Lukistyowati
Department of Aquaculture, Faculty of Fisheries and Marine, Universitas Riau
Agriculture, Biological Sciences & Forestry

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INTESTINE AND LIVER HISTOPATHOLOGY OF STRIPED CATFISH (Pangasianodon hypophthalmus) FEEDING CONTAINING PAPAYA LEAF FERMENTATION Yoshua Demawan Mangapul Purba; Henni Syawal; Iesje Lukistyowati
Asian Journal of Aquatic Sciences Vol 6 No 1 (2023): April 2023
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/ajoas.6.1.50-61

Abstract

Aeromonas hydrophila bacteria often attack internal organs such as the liver and intestines. This research was conducted in June – August 2021 at the Laboratory of Fish Parasites and Diseases, Faculty of Fisheries and Marine, Universitas Riau. Histology preparations were made at the Bukittinggi Veterinary Center. This research aimed to obtain the best dose addition of papaya leaf fermented solution which could reduce intestinal and liver tissue damage of striped catfish (P.hypophthalmus) which was challenged with A.hydrophila. The method used is an experimental method with a completely randomized design (CRD) with 4 treatment levels and 3 replications; Kp (Feed not given papaya leaf fermented solution + infected with A.hydrophila), P1 (16 ml/kg feed of papaya leaf fermented solution + infected with A.hydrophila), P2 (16 ml/kg feed of papaya leaf fermented solution) + infected with A.hydrophila), P3 (16 ml/kg of papaya leaf fermented solution feed + infected with A. hydrophila). The fish used was 8-10 cm with a density of 1 fish/3 L totaling 10 fish with a container measuring 40x30x30 cm. Infection by injection in the intramuscular section as much as 0.1 mL/head with a bacterial density of 108 CFU/mL. The parameters observed were clinical symptoms for 72 hours and histopathology of the intestine and liver of striped catfish. The results showed that the intestine saw the presence of hemorrhage, necrosis, and edema. While the liver shows abnormalities, such as hemorrhage, necrosis, hypertrophy, and fatty degeneration.
HEMATOLOGY OF ASIAN REDTAIL CATFISH (Hemibagrus nemurus) REARED IN MEDIA WITH REGULAR ADDITION OF PROBIOTICS Rona Lestari; Henni Syawal; Iesje Lukistyowati
Asian Journal of Aquatic Sciences Vol 6 No 1 (2023): April 2023
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/ajoas.6.1.10-20

Abstract

Multi-cell probiotic containing bacteria Nitrosomonas sp., Nitrobacter sp., and Bacillus sp. This research was conducted from March to May 2021 at the Fish Disease and Parasite Laboratory, Department of Aquaculture, Faculty of Fisheries and Marine, Riau University. This study aimed to analyze the hematology of Asian redtail catfish reared in media that was added with probiotics periodically and to obtain the best time interval for giving probiotics to the rearing medium. The method used in this study is an experimental method by applying a Completely Randomized Design (CRD) with five treatment levels and three replications. The treatments used were the administration of probiotics at a dose of 0,01 mL/L of water with different time intervals, P0 (without treatment), P1 (5 days once), P2 (10 days once), P3: (15 days once), and P4 (20 days once). Fish were reared for 50 days. The results show that the Asian redtail catfish reared with the addition of probiotics in the rearing medium had a significant effect (P<0.05) with the best time interval being once every 5 days in the P1 treatment, seen from the total erythrocytes 2.43 x 106 cells/mm3, hematocrit level 28.67%, hemoglobin level 7.40 g/dL, total leukocytes 9.92 x 104 cells/mm3, leukocrit level 1.83%, and glucose 49.67 g /dL. Water quality during the study was still in the normal range that could support the growth of Asian redtail Catfish, namely temperature 26-27.1 C, pH 5.8-6.5, DO 6.0-7.4 mg/L, and ammonia 0.0011-6.5 0.0047 mg/L
HISTOPATHOLOGY OF KIDNEY AND INTESTINE OF TILAPIA (Oreochromis niloticus) FED WITH HERB FERMENTED FEED AFTER CHALLENGE OF Streptococcus agalactiae Abdullah Al Furqon; Morina Riauwaty; Iesje Lukistyowati
Asian Journal of Aquatic Sciences Vol. 6 No. 2 (2023): August
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Abstract

The natural ingredients' potential as immunostimulants is a plant Curcuma and Aromatic ginger. This research was conducted from March to June 2022 in the Laboratory of Parasites and Fish Diseases, Faculty of Fisheries and Marine, Universitas Riau. Histological preparations were carried out at the Bukittinggi Veterinary Center (BVet). This research aimed to know the structure of the Kidneys and Intestines of tilapia by adding herb-fermented feed to prevent Streptococcus agalactiae infection. This research was an experimental method using a Completely Randomized Design (CRD) with 4 treatments and 3 replications. This treatment of the study was; NC (were not immersed and infected), PC (were not immersed in fermented herbs solution but infected of S.agalactiae), T1 (Giving 100 mL/kg fermented herbs), T2 (Giving 125 mL/kg fermented herbs), T3 (Giving 150 mL/kg fermented herbs). The fish used were 6 - 7 cm in size and reared with a stocking density of 1 fish/3 L of water and kept for 46 days. Fish were challenged with S.agalactiae bacteria with a density of 108 CFU/mL at a dose of 0.1 mL/head intramuscularly on day 32, after which the fish were reared again until day 46. Changes that occur in the kidneys of fish are hemorrhage, hypertrophy, and necrosis, while in the intestines there are histological changes in the form of haemorrhage, edema, vacuole degeneration, and necrosis. The results show that the feeding containing the herbal medicine of Curcuma and Aromatic ginger fermented affected the improvement of the histological changes in the kidneys and intestines of tilapia
Co-Authors Abdullah Al Furqon Henni Syawal Rona Lestari Yoshua Demawan Mangapul Purba