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Iwa Sutardjo Rus Sudarso

Universitas Gadjah Mada, Yogyakarta, Indonesia

Author-ID : 551898

Dentistry

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Antitumor activity of antisense oligonucleotide p45Skp2 in soft palate carcinoma cell squamous in vitro Supriatno Supriatno; Sartari Entin Yuletnawati; Iwa Sutardjo Rus Sudarso
Dental Journal (Majalah Kedokteran Gigi) Vol. 46 No. 1 (2013): March 2013
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: Human soft palate cancers are characterized by a high degree of local invasion and metastasis to the regional lymph nodes. Treatment options for this cancer are limited. However, a new strategy for refractory cancer, gene therapy is watched with keen interest. p45Skp2 gene as a tumor promoter gene is one of target of the oral cancer therapy. To inhibit the activity of p45Skp2 gene is carried-out the genetic engineering via antisense technique. Purpose: To examine the antitumor activity of p45Skp2 antisense (p45Skp2 AS) gene therapy in human soft palate [Hamakawa-Inoue (HI)] cancer cells. Methods: Pure laboratory experimental study with post test only control group design was conducted as a research design. To investigate the apoptosis induction of p45Skp2 AStransfected cell was evaluated by colorimetric caspase-3 assay and Flow cytometry. Furthermore, to detect the suppression of in vitro HI cell invasion and cell growth of p45Skp2 AS-treatment cell was examined by Boyden chamber kit and MTT assay, respectively. Results: The cell number of p45Skp2 AS-treated HI cell was significant decreased when compared with that of p45Skp2 sense (p45Skp2 S) cells (p<0.05). p45Skp2 AS-treated cell induced apoptosis characterized by an increase in the early and late apoptosis, and activation of caspase-3 (p<0.05). Therefore, suppression of HI cell invasion and cell growth were markedly increased by p45Skp2 AS treatment (p<0.05). Conclusion: Antisense oligonucleotide p45Skp2 has a high antitumor activity in human soft palate cancer cell, targeting this molecule could represent a promising new therapeutics approach for this type of cancer.Latar belakang: Kanker palatum lunak mempunyai karakteristik invasi dan metastasis ke limfonodi regional yang tinggi. Pilihan perawatan kanker tersebut masih sangat terbatas. Walaupun demikian, strategi baru untuk penanganan kanker yaitu terapi gen menjadi pilihan utama. Gen p45Skp2 sebagai gen pemacu tumor merupakan salah satu target terapi kanker oral. Untuk menghambat aktivitas gen p45Skp2 tersebut dilakukan rekayasa genetik melalui teknik antisense. Tujuan: Menguji aktivitas antitumor gen p45Skp2 antisense (p45Skp2 AS) terhadap sel kanker palatum lunak (sel HI). Metode: Jenis penelitian yang digunakan adalah eksperimen laboratorik murni dengan rancangan posttest only control group design. Induksi apoptosis sel yang ditransfeksi p45Skp2 AS dievaluasi menggunakan uji caspase-3 kolorimetrik dan flow cytometry. Untuk mendeteksi hambatan invasi dan pertumbuhan sel HI yang ditransfeksi p45Skp2 AS dilakukan uji Boyden chamber dan uji MTT. Hasil: Pertumbuhan sel HI yang ditransfeksi p45Skp2 AS menurun secara signifikan dibandingkan dengan p45Skp2 Sense (S) (p<0,05). Sel HI transfeksi p45Skp2 AS menginduksi apoptosis dengan meningkatkan aktivitas proteolitik caspase-3 dan early and late apoptosis (p<0,05). Hambatan invasi dan pertumbuhan sel HI secara signifikan meningkat pada sel yang diperlakukan dengan p45Skp2 AS (p<0,05). Kesimpulan: p45Skp2 AS oligonukleotida mempunyai aktivitas antitumor yang kuat pada sel kanker palatum lunak. Target dari molekul tersebut dapat menjanjikan suatu terapeutik baru untuk jenis kanker palatum tersebut.

Correlation between age and dental arch dimension of Javanese children Atiek Driana Rahmawati; Iwa Sutardjo Rus Sudarso; Dibyo Pramono; Eggi Arguni
Dental Journal (Majalah Kedokteran Gigi) Vol. 53 No. 2 (2020): June 2020
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v53.i2.p93-98

Background: Dental arch form and dimension are fundamental factors in orthodontic diagnosis and treatment planning. Its dimension will increase, due to the eruption of teeth, and is also affected by ethnicity, nutrition, systemic disease, hormonal factors, and gender. Many teeth are erupting in 8–10-year-old children. Purpose: This study aimed to assess the correlation between age and dental arch dimension of Javanese children in good nutritional status for consideration of orthodontic treatment. Methods: This was a cross-sectional study with 66 children aged 8–10 years in a normal dentoskeletal relationship, grouped based on age as the subject. Each group consisted of 22 pairs of dental study models, male and female. Anterior and posterior size of dental arch length were measured by digital sliding calipers from the midpoint between the right and left permanent central incisors perpendicular to the inter-canines and inter-molars. The width was measured at the inter-canines and inter-molars. Results: Pearson’s correlation test showed that there were significant correlations between age and maxillary dental arch lengths (p = 0.01, r = 0.31 for anterior, and p = 0.043, r = 0.249 for posterior). Conclusion: Based on this study, it can be concluded that there was a positive correlation between age and dental arch length of 8–10-year-old Javanese children in good nutritional status, especially in maxillary dental arch length.

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