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All Journal AGRIVITA, Journal of Agricultural Science
Slamet Abadi
Department of Agribusiness, Faculty of Agriculture, Universitas Singaperbangsa Karawang, Indonesia
Agriculture, Biological Sciences & Forestry

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Maize Media Enhance the Conidia Production of Entomopathogenic Fungi Lecanicillium lecanii also Its Effective to Control the Weevil Cylas formicarius (Fabricius) (Coleoptera: Brentidae) Lutfi Afifah; Aulia Corry Aena; Nurcahyo Widyodaru Saputro; Anik Kurniati; Rosalia Maryana; Ani Lestari; Slamet Abadi; Ultach Enri
AGRIVITA, Journal of Agricultural Science Vol 44, No 3 (2022)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v44i3.3605

Abstract

The growth of entomopathogenic fungi Lecanicillium lecanii is strongly related to the media in increasing the fungi sporulation, enhancing its effectiveness in infecting target pests. This study aims to obtain L. lecanii propagation media with the highest conidia production, and the increased virulence on Cylas formicarius. The first stage of alternative media selection consists of: Potato Dextrose Agar; maize, rice; bran. The second stage of infectivity of the best alternative media consists of 5 treatments in 5 replications: synthetic insecticide (C+); distilled water (C-); 107 conidia/ml; 108 conidia/ml; 109 conidia/ml. The results show that the best colony growth rate is (1.15 mm) on Maize media, and the highest conidia density is (4.2 x 10⁸ conidia/ml) on Maize media is not significantly different from PDA (1.3 x 10⁸ conidia/ml). The best germination rate is (74.31%) on Maize media, and the highest media weight (1.10 g) on Rice media is not significantly different from maize (1.45 g). The infectivity of L.lecanii affect the mortality of C. formicarius (74%) substantially at a concentration of 109 conidia/ml. The LC50 value obtained is 2.6 x 10⁷ conidia/ml. Thus, maize media can be an alternative medium for mass propagation of L. lecanii.
Co-Authors Ani Lestari Anik Kurniati Aulia Corry Aena Lutfi Afifah Nurcahyo Widyodaru Saputro Rosalia Maryana Ultach Enri