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All Journal HAYATI Journal of Biosciences Indonesian Journal of Biotechnology TEKNOLOGI PANGAN : Media Informasi dan Komunikasi Ilmiah Teknologi Pertanian
Rachma Wikandari
Departemen Teknologi Pangan Dan Hasil Pertanian, Fakultas Teknologi Pertanian, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Immunology & microbiology Materials Science & Nanotechnology

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Cold Stress Response Genes of Lantiplantibacillus plantarum subsp. plantarum Mut-3 and Lantiplantibacillus plantarum subsp. plantarum Mut-7 Support the Ability to Survive in Low-Temperature Conditions Eunike Marganingrum Andriani Samodra; Dian Suroto; Tyas Utami; Rachma Wikandari; Endang Sutriswati Rahayu
HAYATI Journal of Biosciences Vol. 30 No. 1 (2023): January 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.1.65-70

Abstract

Probiotics are widely consumed in various food matrices to provide health benefits to the host. The viability of probiotic cells is influenced by several factors, including exposure to high temperatures during the production process and low temperatures during storage. In this study, we report the response to cold stress of Lantiplantibacillus plantarum subsp. plantarum Mut-3 and Mut-7 after 24 h of storage at 4°C and -20℃. The cell number of Lantiplantibacillus plantarum subsp. plantarum Mut-3 and Mut-7 in low-temperature condition had no significant differences than their initial number: 11.88 log CFU/ml and 11.62 log CFU/ml at 4°C; 11.51 log CFU/ml and 11.47 log CFU/ml at -20°C for Mut-3 and Mut-7 respectively. The results indicated the survival capacity of Lantiplantibacillus plantarum subsp. plantarum Mut-3 and Mut-7 at low temperatures. The genes encoding cold shock proteins for the response to cold stress were evaluated by genome sequencing. The CspA/CspC genes of Lantiplantibacillus plantarum subsp. plantarum Mut-3 and Mut-7 possibly play a role in maintaining cell resistance at low temperatures, since the genes products predicted to have conserved motifs in the RNA binding protein (RNP) -1 and RNP-2 responsible for cold response stress which are similar to those in other bacteria.
Penerapan panduan produksi dan distribusi pada kondisi darurat pandemi pada industri jasaboga kategori A1, A2, dan A3 di Yogyakarta Andika Wicaksono Putro; Marsellina Tirza Setiawan; Ahmad Lutfi Karim; Rachma Wikandari; Endang S. Rahayu

Publisher : Program Studi Ilmu dan Teknologi Pangan, Universitas Yudharta, Pasuruan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35891/tp.v14i1.2801

Abstract

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(????1, ????2, ???????????? ????3) ???????? ????????????????????????????????????????. ???????????????? ???????????????? ???????????????????????????????? ???????????????????????????? ???????????????????????????????????????????? ???????????? ???????????????????????????????????????? ???????????????????? ???????????????????????????????????????????????????????? ???????? 60 ???????????????????????????????????? ???????? ????????????????????????????????????????. ???????????? ???????????????????????????????? ???????????????????????????? ???????????????????????????????? ???????????????????????????????? ????3 ???????????? ???? ????????????????????????????-???????????????????????????????????????? ???????????????????? 70.07 ???????????? ???????? 88 ???????????????? “???????????????? ????????????????” ????????????????????????????????. ???????????? ???????????????? ???????????????????????????? ???????????????????????????????? ???????? ???????????????????????????????????????? ????1 ???????????? ????2 ???????????? ???? ???????????????????? ???????? 39.67 ???????????? 47.11 ???????? ???????????? ???????????????????????????????? ???????? ????????????????????????????-????????????????????????????????????????, ???????????????????? ???????????? ???????????????????????????????????????????? ???????? “???????????????????? ????????????????”. ???????????? ???????? ???????????? ???????????????????? ???????????????????????????? ???????????????????????????? ???????????????????????????????????????? ???????????? ???????????????????????????????????????? ???????? ???????????? ???????????????????? ???????? ???????????? ???????????????????????????????? ???????? ????????????????????????????-????????????????????????????????????????, ???????????????????????????????????? ???????????? ????????????????????’???? ???????????????????????????????????? ???????? ???????????? ????????????????????????????-???????????????????????????????????????? ????????????????, ???????????? ????????????????????’???? ???????????????????????????????????? ???????? ????????????????????????????-???????????????????????????????? ????????????????????????????????????????????, ???????????? ????????????????????'???? ???????????????????????????????????????? ???????? ???????????????????????????????????? ???????????? ????????????????????????????-???????????????????????????????????????? ????????????????????????????????????????????????????, ???????????? ???????????????????????? ???????? ???????????????????????????????? ????????????????????????????????????, ???????????? ???????????????????????????????? ???????????????????? ????????????????????????, ???????????? ???????????????????????????????????? ???????? ???????????????????????????????? ???????????????????????????????? ????????????????????????????. ???????????????? ???????????????????? ????????????????????????????, ???????? ???????????????????????????? ???????????? ???????????????????????????????? ???????? ????????????????????????????-????????????????????????????????????????, ???????? ???????? ???????????????????????????????????? ???????? ???????????????????????????????????????????? ???????????????????????????????????????????? ???????? ???????????????? ???????????????????????????????????????????????????? ???????????????????????????????????? ???????????? ???????????????????????????????????????????? ???????????????????????????? ???????? ???????????? ???????????????????????????????? ???????? ????????????????????????????-????????????????????????????????????????.
In silico analysis of antibiotic resistance genes in Lactiplantibacillus plan‐ tarum subsp. plantarum Kita‐3 Angelia Wattimury; Dian Anggraini Suroto; Tyas Utami; Rachma Wikandari; Endang Sutriswati Rahayu
Indonesian Journal of Biotechnology Vol 28, No 2 (2023)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.72550

Abstract

The absence of transferable antibiotic resistance genes is required for the safety of commercial probiotics. Previous studies have found that antibiotic resistance genes on plasmids in Lactobacilli make them unsafe for food purposes due to the genes’ ability to transfer to pathogenic microorganisms. In contrast, bacteria from the Lactobacillaceae family are widely used as a probiotic. This study assessed the antibiotic susceptibility of Lactiplantibacillus plantarum subsp. plantarum Kita‐3 (previously known as Lactobacillus plantarum K‐3) isolated from Halloumi cheese using eight antibiotics. Genome sequencing was performed using the Illumina NovaSeq 6000 sequencing platform to detect the presence of antibiotic resistance genes on chromosomes and plasmids. L. plantarum subsp. plantarum Kita‐3 was resistant to clindamycin, streptomycin, and chloramphenicol but susceptible to tetracycline, ampicillin, kanamycin, erythromycin, and ciprofloxacin. Genome sequencing of L. plantarum subsp. plantarum Kita‐3 verified the presence of tetracycline, fluoroquinolones, β‐lactamase resistance genes, and multidrug resistance efflux. Kita‐3 had no transposable elements, gene transfer agents, plasmid‐related functions, or intact prophages. Overall, this study produced the antibiotic resistance profile of L. plantarum subsp. plantarum Kita‐3 to assess the risk of transferring antibiotic resistance genes to other bacteria. The study provides essential data on the safe use of L. plantarum subsp. plantarum Kita‐3 as probiotics.
Co-Authors Ahmad Lutfi Karim Andika Wicaksono Putro Angelia Wattimury Dian Anggraini Suroto Dian Suroto Endang S. Rahayu Endang Sutriswati Rahayu Eunike Marganingrum Andriani Samodra Marsellina Tirza Setiawan Rahayu, Endang Sutriswati Tyas Utami Tyas Utami