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Belkheir Hammouti
University Mohammed Premier
Computer Science & IT Environmental Science

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Artemisia herba alba Essential Oil: GC/MS analysis, antioxidant activities with molecular docking on S protein of SARS-CoV-2 Khaoula Diass; Imane Oualdi; Mohammed Dalli; Salah-eddine Azizi; Mounir Mohamed; Nadia Gseyra; Rachid Touzani; Belkheir Hammouti
Indonesian Journal of Science and Technology Vol 8, No 1 (2023): IJOST: April 2023
Publisher : Universitas Pendidikan Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17509/ijost.v8i1.50737

Abstract

This study was conceived to work on Artemisia herba alba essential oils (EOs) originating from Eastern Morocco. We investigated the chemical properties and determined the antiradical scavenging activity. The GC-MS technique was used to elucidate the chemical composition of the two studied regions. Jerada Eo was distinguished by the existence of 1,6-Dimethylhepta-1,3,5-triene (36.44%), Camphor (22.50%), and Thujone (7.21%). While, Taourirt EO was found to be rich in Camphor (55.31%), Eucalyptol (14.64%), and Camphene (9.95%). Moreover, the free radical scavenging ability (DPPH•), and ferric reducing power assay (FRAP) were conducted. The EOs tested are found to be weakly active and to have important antioxidant potential. The docking study was also carried out by theoretical investigation with molecular docking of four active predominating components (1,6-dimethylhepta-1,3,5-triene; Chrysanthone; Eucalyptol; Alpha-pinene) against S protein of SARS-CoV‑2, and all the four ligands were found to bind to the S protein 6CS2 of SARS-CoV-2 differently and that the stable complex formed as a result of these interactions may prevent the binding of ACE2 with the spike S protein of SARS-CoV-2.
Technological Aptitude and Sensitivity of Lactic Acid Bacteria Leuconostoc Isolated from Raw Milk of Cows: From Step-by-Step Experimental Procedure to the Results Nora Hamdaoui; Yahya Rokni; Abdeslam Asehraou; Mohamed Mouncif; Zakaria Mennane; Abdelouadoud Omari; Asmae Sellam; Belkheir Hammouti; Mustapha Meziane
Indonesian Journal of Science and Technology Vol 8, No 2 (2023): (ONLINE FIRST) IJOST: September 2023
Publisher : Universitas Pendidikan Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17509/ijost.v8i2.53730

Abstract

We demonstrated several technological aptitudes and sensitivity of lactic acid bacteria (Leuconostoc) isolated from the raw milk of cows. We explained the experiments in detail to get the reader to understand what kinds of technology can be used for testing bacteria. Lactic acid bacteria are used in the production of dairy products to increase their shelf life and improve their organoleptic and nutritional properties. Leuconostocs is a heterofermentative bacterium that produces lactic acid, acetic acid or ethanol, and carbon dioxide. A collection of 138 strains of lactic acid bacteria were isolated from the raw milk of cows in the region of eastern Morocco. 38 strains were identified as Leuconostocs. Their subspecies are 16 Mesenteroides, 11 Dextranicum, and 11 Cremoris. Our study was interested in the technological aptitude of this genus looking for the production of dextran and growth in milk with additional sunflower dye, as well as the acidifying, flavoring, thickening, coagulating, proteolytic activity in solid medium and Lipolytic activity. This work also consisted of studying the profile of the resistance and the sensitivity of these strains to antibiotics.
Study of the Catecholase Activity of new catalysts Based on Copper (II) and Heterocyclic Ligands Rafika El Ati; Haytham Bouammali; Mohamed El Kodadi; El Bekkay Yousfi; Rachid Touzani; Belkheir Hammouti
Indonesian Journal of Science and Technology Vol 7, No 1 (2022): IJOST: VOLUME 7, ISSUE 1, April 2022
Publisher : Universitas Pendidikan Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17509/ijost.v7i1.41605

Abstract

In this work, we are interested in finding new catalysts for catecholase, whose principle is based on the oxidation reaction of catechol to o-quinone. We approached the synthesis of the threes tripod ligands based on pyrazole in a condensation reaction and its characterization by IR, 13C NMR, 1H NMR spectroscopy, then we evaluated the catalytic properties of certain complexes formed in situ to catalyze the oxidation reaction of catechols to o-quinones. The aim is to find the right models to reproduce the catalytic activity of the enzyme (catecholase), we used complexes formed in situ by pyrazole derivatives with Copper salts. Among these complexes, the complex L4/Cu(CH3COO)2 showed good catalytic activity of the combination (1ligand/2metal) in MeOH for this reaction, with a speed Vmax equal to 69.38 μmol.L-1.min-1 and a low value of Km equal to 0.019 mol.L-1. We have demonstrated that the nature of concentration, ligand, solvent, and copper salts, influenced strongly the catecholase activity.
Co-Authors Abdelouadoud Omari Abdeslam Asehraou Asmae Sellam El Bekkay Yousfi Haytham Bouammali Imane Oualdi Khaoula Diass Mohamed El Kodadi Mohamed Mouncif Mohammed Dalli Mounir Mohamed Mustapha Meziane Nadia Gseyra Nora Hamdaoui Rachid Touzani Rafika El Ati Salah-eddine Azizi Yahya Rokni Zakaria Mennane