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All Journal Jurnal Kimia Riset Indonesian Journal of Pharmaceutical Research
Riong Seulina Panjaitan
Fakultas Farmasi, Universitas 17 Agustus 1945 Jakarta
Chemistry Medicine & Pharmacology Public Health

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ANTIOXIDANT AND TOXICITY ACTIVITIES of Gracilaria gracilis METHANOL EXTRACT BASED ON DIFFERENT EXTRACTION METHODS Riong Seulina Panjaitan; Windri Yuliana
Jurnal Kimia Riset Vol. 7 No. 2 (2022): December
Publisher : Universitas Airlangga, Campus C Mulyorejo, Surabaya, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jkr.v7i2.38255

Abstract

Gracilaria gracilis (red macroalgae) was collected from Sayang Heulang Beach, Garut, West Java. It has a number of secondary metabolites that potentially serve as a source of natural antioxidants. The objective of this study was to compare the optimal extraction method in particular time variation for producing the highest percent of yield, antioxidant activity, and toxicity of G. gracilis methanol extract. This study was conducted experimentally, including macroalgae sampling and preparation, phytochemical screening, extraction of G. gracilis by maceration and UAE methods, antioxidant activity testing based on the DPPH test (2.2 diphenyl-1-picrylhydrazyl) and toxicity testing using the BSLT (Brine Shrimp Lethality) method. The results indicated that the methanol extract of G. gracilis contains flavonoids and steroids, with yield percentages of 12.93% for maceration and 12.1% for UAE. The antioxidant activity (IC50) of maceration was 86.46 ppm, whereas the UAE was 59.01 ppm. Then, the toxicity test (LC50) for maceration was 28.35 ppm whereas the UAE was 27.76 ppm. Macerated methanol extract and UAE G. gracilis have the potential to be powerful antioxidants based on their IC50 values. Then, macerated methanol extract and UAE are included in the highly toxic category.
Carbohydrate Content in Packaged Chicken Nuggets: Qualitative And Quantitative Analysis Syifa Alifia Indriani; Nurul Asfia; Agnes Manongga; Riong Seulina Panjaitan
Indonesian Journal of Pharmaceutical Research Vol 2, No 2 (2022): Indo J Pharm Res 2022 2(2)
Publisher : Department of Pharmacy, Muhammmadiyah University of Sumatera Barat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31869/ijpr.v2i2.4174

Abstract

Nugget products can be made from beef, chicken, fish, and other ingredients, but chicken nuggets are the most popular in the community. Chicken nuggets are products produced from the marinated, ground, minced, and cooked parts of chicken breast meat with breadcrumbs. Qualitative and quantitative tests of carbohydrates are carried out to determine the presence of carbohydrates present in nuggets. Qualitative tests are based on changes in color or deposits formed, while quantitative tests to determine the carbohydrate levels contained in each test material with DNS method. The results showed qualitative analysis of molisch test carbohydrates on samples of chicken meat nuggets brand "B," which positively contained carbohydrates (glucose), while samples of chicken meat nuggets brands "A" and "C" showed negative results. In the Iodin test, all three nugget samples showed positive results for glycogen. The Benedict test yielded negative results for all three nugget samples, indicating that none of them contained sucrose. The three nugget samples all failed the Barfoed test, indicating that they are all members of the disaccharide group. The Seliwanoff test yielded negative results for all three nugget samples, all of which contained glucose, mannose, or galactose. The Osazon test yielded negative results for all three nugget samples. Carbohydrate quantitative test obtained sample concentration on sample nugget of chicken meat brand "A" 0.49 mg/mL, sample of chicken meat nugget brand "B" 0.48 mg/mL and sample nugget of chicken meat brand "C" 0.56 mg/mL. Carbohydrate qualitative analysis on samples of chicken meat nuggets from brand "B" reveals that they positively contain carbohydrates as well as fructose and glycogen. The "C" brand chicken meat nugget sample has a high concentration for quantitative analysis of carbohydrates, indicating that the sample contains more carbohydrates.
Co-Authors Agnes Manongga Nurul Asfia Syifa Alifia Indriani Windri Yuliana