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All Journal HAYATI Journal of Biosciences
Pongsakorn Sunvittayakul
Department of Genetics, Faculty of Science, Kasetsart University, Bangkok, Thailand
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences

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Carbon-nanotube for Transient Expression in Rice Calli Paphawarin Pinyokham; Kamolwan Khianchaikhan; Pongsakorn Sunvittayakul; Supachai Vuttipongchaikij; Piyama Tasanasuwan; Chatchawan Jantasuriyarat
HAYATI Journal of Biosciences Vol. 29 No. 6 (2022): November 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.6.845-850

Abstract

Transient gene expression is an important technique in gene functional analysis, protein production and in plants. However, traditional transient expression methods using Agrobacterium are time-consuming with low efficiency. In this study, we demonstrated the use of a single-walled carbon nanotube (SWCNT) to deliver 35S:mCherry:pCXSN plasmid into rice calli. This transient expression protocol used a plastic medical syringe to create the physical pressure to help the delivery of plasmid DNA into plant cells. This protocol is relatively easy to perform and low cost. The transient expression was observed under fluorescence microscopy, and the mCherry fluorescence signal was quantified. The plasmid DNA was delivered into the rice cell using a 3:1 ratio (plasmid: carbon nanotube). The result showed that the mCherry signal of carbon nanotube + plasmid DNA treatment was the highest signal at 3 days post-transformation and decreased to a lower signal at 6 days post-transformation. Moreover, the quantitative analysis of mCherry mean intensity revealed that the signal intensity of carbon nanotube + plasmid DNA treatment was the highest level, and significantly higher than the control treatments at 3 days post-transformation. Plasmid DNA can be transported easily into plant calli using this carbon nanotube transient expression protocol.
Co-Authors Chatchawan Jantasuriyarat Kamolwan Khianchaikhan Paphawarin Pinyokham Piyama Tasanasuwan Supachai Vuttipongchaikij