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All Journal HAYATI Journal of Biosciences
Edward Ciputra
Department of Biomedicine, School of Life Sciences, Indonesia International Institute for Life Sciences, Jakarta Timur, Indonesia
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences

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The Potential Selective Oncolytic Effects of Newcastle Disease Virus (NDV) on Cervical and Colon Cancer Cell Lines Jyothsna Girish; Lorina Handayani Ulag; Fabian Marcello; Alvaro Filbert Liko; Nathaniel Alvin Sanjaya; Edward Ciputra; Sharleen Elvira; Kenny Yonatan; Monica Angel Tanissa; Yosua Nungky Setiawan; Felic Fanusi; Marsia Gustiananda
HAYATI Journal of Biosciences Vol. 30 No. 1 (2023): January 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.1.105-112

Abstract

The mesogenic and velogenic strains of the avian Newcastle Disease Viruses (NDV) have been investigated for use in cancer virotherapy. However, despite its promising results, these strains have the potential to cause outbreaks leading to morbidity and mortality in large numbers of avians that can devastate the poultry industry. Therefore, there is a growing interest towards the use of lentogenic strains such as NDV LaSota vaccine strain for virotherapy. This study investigated the oncolytic potential of the NDV LaSota strain on colorectal cancer (HT29) and cervical cancer (HeLa) cell lines. The NDV LaSota stain was propagated in pathogen free embryonated chicken eggs and quantified using a hemagglutination assay. The oncolytic activity was evaluated by comparison of HT29 and HeLa cell lines to the non-cancerous human embryonic kidney (HEK293) cell line using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) viability assay. The highest NDV concentration of 16 HAU was found to reduce the viability of HT29, HeLa, and HEK293 cell lines to 42±3% (p = 0.0001), 49±12% (p = 0.0009), and 77±5% (p = 0.007), respectively, indicating its potential selective cytotoxicity, as supported by the disturbed cell morphology. The NDV LaSota strain exhibits a potential selective oncolytic activity towards cancer cell lines, thus may serve as an interesting candidate for virotherapy against colorectal and cervical cancer.
Co-Authors Alvaro Filbert Liko Fabian Marcello Felic Fanusi Jyothsna Girish Kenny Yonatan Lorina Handayani Ulag Marsia Gustiananda Monica Angel Tanissa Nathaniel Alvin Sanjaya Sharleen Elvira Yosua Nungky Setiawan