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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences
Bhupal Govinda Shrestha
Kathmandu University
Agriculture, Biological Sciences & Forestry Environmental Science

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Study of Phytochemical, Anti-Microbial, Anti-Oxidant, Phytotoxic, and Immunomodulatory Activity Properties of Bauhinia variegata Jaya Bhandari; Pratiksha Thapa; Prasodhan Niraula; Nita Thapa; Nikita Shrestha; Bhupal Govinda Shrestha
Journal of Tropical Life Science Vol. 7 No. 1 (2017)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.07.01.14

Abstract

There is growing interest in the use plants for the treatment and prevention of cancer and are currently being evaluated as promising anticancer agents. In this paper, we investigated the anti-microbial, anti-oxidant, phytotoxic and immunomodulatory of plant Bauhinia variegata and also established the presence of important phytoconstituents which might signify its anticancer property. Phytochemical screening, anti-microbial assay, phytotoxicity assay, anti-oxidant assay and immunomodulatory analysis was done according to the protocol established at Department of Biotechnology, Kathmandu University and Panjwani Center for Molecular Medicine and Drug Research, University of Karachi, Pakistan.In the phytochemical screening, we observed the presence of different phytochemicals like steroids, terpenoids, flavonoids, reducing sugars and glycosides. In case of Antimicrobial assay, the plant was found to be most effective against B.cereus with the highest zone of inhibition (ZOI) of 12mm and against Rhizopus with ZOI of 11mm. During the antioxidant assay in comparison to Ascorbic acid; at highest concentration, the scavenging activity shown by the plant Bauhinia variegata are 43.38% as compared to ascorbic acid 89.25%. In the Immunomodulatory assay at the highest concentration i.e. 250 µg/ml, the plant showed 75.1% effect, which showed that the plant has potential anti-inflammatory potential. In phytotoxicity assay, Bauhinia variegata showed 20% phytotoxicity. Bauhinia variegata has thus been proven to be an important candidate to be used as an antimicrobial, antioxidant, anti-inflammatory, phytotoxic and anticancer agent. Separation of pure compounds with bioassay guided extraction, spectrometric analysis and subsequent cytotoxicity assay of the pure bioactive compounds from Bauhinia variegate is highly recommended as crude extract itself showed promising phytotoxicity and other pharmaceutical potential.
Study of Serum Lactate Dehydrogenase and Gamma-Glutamyl Transpeptidase in Breast Cancer Patients receiving Chemotherapy. Anil Singh Basnyat; Abimanyu Jha; R Pathak; Bhupal Govinda Shrestha
Journal of Tropical Life Science Vol. 7 No. 2 (2017)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.07.02.07

Abstract

Breast cancer (BC) is the most common type of cancer worldwide, being a major cause of morbidity in female. In Nepal it is the second most common type of cancer among females of perimenopausal age group. More than one quarter of the BC diagnosed in young Nepalese female, with familial history of breast cancer, early pregnancy, longer lactation and estrogen exposure and often with tumors showing aggressive biological behaviors. Anthracyclines (Doxorubicin) based treatment regime were reported to cause cardiotoxicity by increasing intramyocardial free radical production, lipid alterations and decreasing antioxidant level. Oxidative stress involving cellular reactive oxygen species (ROS) production is widely accepted mechanism but the molecular basis of chemotherapy induced organ toxicity remains highly controversial. An increased rate of metabolism and oxidative stress results rapid turnover of cancer cells that modulates the enzyme level in blood circulation. Serum LDH and GGT level correlates with tumor burden, metastatic character of BC and intensity of organtoxicity. The aim of our study is to evaluate the serum level of LDH and GGT in BC patients receiving chemotherapy and correlate these enzyme levels with different stages of BC. A total number of 150 subjects were included in the study, comprising 90 histopathologically confirmed 24 to 76 years aged patients of different breast cancer stages, receiving at least 3 cycles of 5-Fluorouracil, Adriamycin and Cyclophosphamide (FAC) chemotherapy. Sixty age- matched healthy women were enrolled as controls. Blood samples from each individual was collected after informed consent and analysed for serum LDH and GGT levels using standard biochemical methods. Data were analyzed using student's paired 't' test, Pearson correlation test and ANOVA. Serum LDH and GGT levels were significantly (p<0.001) increased in BC patients as compared to control group. When all 4 stages of BC were compared to control group, LDH and GGT showed steady and progressive increase in the activities of these enzymes from stage I-IV. The study concludes that serum LDH and GGT may be sensitive, specific and cost effective biomarkers in early diagnosis of breast cancer, assessing cancer prognosis and response to treatment.
Co-Authors Abimanyu Jha Anil Singh Basnyat Jaya Bhandari Nikita Shrestha Nita Thapa Prasodhan Niraula Pratiksha Thapa R Pathak