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Diah A. Purbaningrum
Universitas Diponegoro

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Effect of Soaking in Chicken Eggshell Solution on Tooth Enamel Hardness Mila D. Yurisya; Diah A. Purbaningrum; Donna Hermawati; Gloria Fortuna
e-GiGi Vol. 10 No. 2 (2022): e-GiGi
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35790/eg.v10i2.40152

Abstract

Abstract: Eggshell is one of the natural wastes which if not treated properly can cause environ-mental pollution. Broiler eggshells consist mostly of CaCO3 (calcium carbonate) that can be synthesized into CaO (calcium oxide) then be processed into Ca (calcium). Calcium has a function to fill the density of bones and teeth that affects their strength and hardness. This study aimed to determine the effect of soaking in broiler chicken eggshell solution on the hardness of tooth enamel. This was an experimental laboratory study with a posttest only control group design. Samples were 24 maxillary first premolar obtained from dental clinics, public health centers, and hospitals in Semarang, Indonesia. Samples were divided into four groups: control group and treatment groups 1, 2, and 3 (duration of soaking in eggshell solution for 2, 7, and 14 days. respectively). The one way ANOVA test resulted in a p-value of 0.000 which meant that there were significant differences in the four groups. The post hoc test showed a p-value of <0.05, which meant that there was a significant difference between the control group (artificial saliva) and the treatment groups of 2.7 and 14 days. In conclusion, chicken eggshell solution has an effect on increasing the hardness of tooth enamel.Keywords: tooth enamel; hardness of tooth enamel; chicken eggshell  Abstrak: Cangkang telur merupakan salah satu limbah alam yang apabila tidak diolah dengan baik dapat  menyebabkan pencemaran lingkungan. Komposisi cangkang telur ayam ras sebagian besar terdiri dari CaCO3 (kalsium karbonat) yang dapat disintesis menjadi CaO (kalsium oksida) dan selanjutnya diolah menjadi Ca (kalsium). Kalsium memiliki fungsi untuk mengisi kepadatan tulang dan gigi yang memengaruhi kekuatan serta kekerasannya. Penelitian ini bertujuan untuk mengetahui pengaruh perendaman larutan cangkang telur ayam ras terhadap kekerasan enamel gigi. Jenis penelitian ialah eksperimental laboratorik dengan posttest only control group design. Sampel penelitian ialah 24 gigi premolar satu rahang atas yang didapatkan dari klinik praktek dokter gigi, puskesmas dan rumah sakit di Kota Semarang. Sampel dibagi atas empat kelompok: kelompok kontrol dan kelompok perlakuan 1, 2, dan 3 (perendaman dalam larutan cangkang telur ayam ras selama 2, 7, dan 14 hari berturut). Hasil uji ANOVA one way memperoleh nilai p=0,000 yang berarti bahwa terdapat perbedaan bermakna pada keempat kelompok. Uji post hoc menunjukkan nilai p<0,05 yang berarti terdapat perbedaan bermakna antara kelompok kontrol (perendaman saliva artificial) dan kelompok perlakuan (perendaman larutan cangkang telur ayam ras 2, 7 dan 14 hari). Simpulan penelitian ini ialah perendaman dalam larutan cangkang telur ayam ras berpengaruh dalam peningkatan kekerasan enamel gigi.Kata kunci: enamel gigi; kekerasan enamel gigi; cangkang telur ayam ras
Aplikasi Sinar Ultraviolet C terhadap Pertumbuhan Koloni Candida albicans Tira H. Skripsa; Gunawan Wibisono; Diah A. Purbaningrum; Yoghi B. Prabowo; Rizky M. Boedi
e-GiGi Vol. 14 No. 2 (2026): e-GiGi
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35790/eg.v14i2.64794

Abstract

Abstract: Healthcare-associated infections (HAIs) often involve Candida albicans as the dominant pathogen. Various sterilization and disinfection procedures using autoclaves, chlorhexidine gluconate, alcohol, and ultraviolet exposure can be employed to reduce the risk. This study aimed to evaluate the effectiveness of Ultraviolet C (UV-C, 254 nm) in reducing the number of C. albicans colonies. This was an experimental laboratory study using a pre- and post-test control group design. A total of 120 Petri dishes containing C. albicans cultures were divided into three groups (one control and two treatment groups with varying durations of UV-C exposure). Colony reduction was calculated manually by comparing the control group with the treatment groups. Analysis was conducted on 1×10⁴ CFU/mL and 1×105 CFU/mL suspensions. The results of the One-Way ANOVA showed a significant difference in the reduction of C. albicans colonies among the treatment groups. The longer the UV-C exposure duration, the greater the observed colony reduction. These findings reinforce the effectiveness of UV-C (254 nm) in reducing the viability of C. albicans with a duration-dependent response pattern. In conclusion, exposure to UV-C light (254 nm) is proven effective in reducing the number of Candida albicans colonies. The longer the duration of exposure, the greater the reduction in the colony count. Keywords: Candida albicans; ultraviolet C   Abstrak: Infeksi terkait pelayanan kesehatan (HAIs) kerap melibatkan Candida albicans sebagai patogen dominan. Terdapat berbagai prosedur sterilisasi dan disinfeksi menggunakan autoklaf, klorheksidin glukonat, alkohol, dan paparan ultraviolet untuk menekan risiko tersebut. Penelitian ini bertujuan untuk mengevaluasi kemampuan sinar ultraviolet spektrum C (UV-C, 254 nm) menurunkan jumlah koloni C. albicans. Jenis penelitian ialah eksperimental laboratorik dengan rancangan pre- and post-test control group. Sebanyak 120 cawan Petri berisi biakan C. albicans dibagi menjadi tiga kelompok (satu kontrol dan dua perlakuan dengan variasi durasi paparan UV-C). Penurunan jumlah koloni dihitung secara manual dengan membandingkan kelompok kontrol dan kelompok perlakuan. Analisis dilakukan pada suspensi 1×10⁴ CFU/mL dan 1×105 CFU/mL. Hasil One-Way ANOVA menunjukkan perbedaan penurunan jumlah koloni C. albicans yang bermakna antar kelompok perlakuan. Semakin lama durasi paparan UV-C, semakin besar reduksi koloni yang diamati. Temuan ini menguatkan efektivitas UV-C (254 nm) dalam menurunkan viabilitas C. albicans dengan pola respons bergantung durasi paparan. Simpulan penelitian ini ialah paparan sinar UV-C (254 nm) terbukti efektif menurunkan jumlah koloni Candida albicans. Semakin lama durasi paparan, semakin banyak jumlah koloni yang berkurang. Kata kunci: Candida albicans; ultraviolet C (UV-C); healthcare-associated infections