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Isolasi Bakteri Pelarut Fosfat Genus Pseudomonas dari Tanah Masam Bekas Areal Perkebunan Karet di Kawasan Institut Teknologi Sumatera Asril, Muhammad; Lisafitri, Yuni
Jurnal Teknologi Lingkungan Vol. 21 No. 1 (2020)
Publisher : Center for Environmental Technology - Agency for Assessment and Application of Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (324.532 KB) | DOI: 10.29122/jtl.v21i1.3743

Abstract

ABSTRACTPhosphorus (P) is a nutrient that is needed by plants. The availability of this element is greatly influenced by soil pH. As for ultisol soils classified as acid soils, most of the P in the soil is not available and is bound to Fe and Al. Pseudomonas, a phosphate solubilizing bacteria are soil microbes that can improve the availability of P in acid soils. This study aims to obtain Pseudomonas indigenous, a phosphate solubilizing bacteria from the acid soil formerly used by rubber plantations in the Institut Teknologi Sumatera. The study was conducted from April to June 2018 which included soil chemical analysis, isolation of the genus Pseudomonads on specific media, testing of phosphate solubility on solid Pikovskaya medium and simple pathogenicity test on potato tubers. The results showed that the sample soil was acidic with a pH of 4.09 with a P-availability of 0.78 ppm. From the soil samples, four potential isolates were obtained from the genus Pseudomonas, namely GSP 01, GSP 13, GSP 15 and GSP 06, with phosphate solubility indexes of 0.885, 0.639, 0.619 and 0.568, respectively. Isolates have the best phosphate solubilizing index on days 4 through 7. The four potential isolates are not pathogenic, so they can be used as isolates to improve the availability of soil nutrients, especially phosphorus needed by plants.Keywords: acid soil, phosphate solubilizing bacteria, phosphate availability, PseudomonasABSTRAKFosfor (P) merupakan unsur hara yang sangat dibutuhkan oleh tanaman. Ketersediaan unsur ini sangat dipengaruhi oleh pH tanah. Pada jenis tanah ultisol yang digolongkan sebagai tanah masam, sebagian besar dari P di tanah dalam bentuk yang tidak tersedia untuk diserap oleh tanaman dan berikatan dengan Fe dan Al. Pseudomonas pelarut fosfat merupakan mikroba tanah yang dapat memperbaiki ketersediaan P pada tanah masam. Penelitian ini bertujuan untuk mendapatkan Pseudomonas pelarut fosfat indigenous dari tanah masam bekas lahan perkebunan karet di kawasan Institut Teknologi Sumatera. Penelitian dilaksanakan pada bulan April sampai Juni 2018 yang meliputi analisis kimia tanah, isolasi bakteri genus Pseudomonads pada medium spesifik, uji kemampuan pelarutan fosfat pada medium Pikovskaya padat serta uji patogenitas sederhana pada umbi kentang. Hasil penelitian menunjukkan bahwa tanah sampel bersifat masam dengan pH 4,09 dengan P tersedia sebesar 0,78 ppm. Dari sampel tanah diperoleh empat isolat potensial yang diperoleh merupakan genus Pseudomonas yaitu GSP 01, GSP 13, GSP 15 dan GSP 06, dengan indeks pelarutan fosfat berturut-turut sebesar 0,885, 0,639, 0,619 dan 0,568. Isolat memiliki indeks pelarutan fosfat terbaik pada hari ke-4 hingga hari ke-7. Keempat isolat potensial tidak bersifat patogen sehingga mampu dijadikan sebagai isolat yang dapat digunakan untuk memperbaiki ketersediaan unsur hara tanah terutama fosfor yang dibutuhkan oleh tanaman.Kata kunci: bakteri pelarut fosfat, fosfat tersedia, Pseudomonas, tanah masam 
UJI EFEKTIVITAS CACING TANAH, KOTORAN SAPI DAN EM4 TERHADAP PENGOMPOSAN SERBUK GERGAJI KAYU JATI Mufti, Aulia Annas; Harliyanti, Putri; Lisafitri, Yuni
Sustainable Environmental and Optimizing Industry Journal Vol 3, No 1 (2021)
Publisher : Universitas Sahid

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36441/seoi.v3i1.327

Abstract

Di Kota Metro, Provinisi Lampung banyak terdapat limbah serbuk gergaji kayu jati. Hal tersebut dikarenakan semakin berkembangnya kerajinan kayu. Seringkali penanganan Limbah serbuk gergaji kayu jati dilakukan dengan cara di bakar. Hal itu akan menimbulkan dampak negatif terhadap lingkungan seperti pencemaran udara. Salah satu alternatif untuk mengurangi limbah serbuk gergaji yaitu dengan cara pengomposan. Penelitian ini dilakukan berdasarkan variasi perlakuan V1: 0,5 kg serbuk gergaji + 0,5 kg kotoran sapi + 500 ml air, V2 : 0,5 kg serbuk gergaji + 0,5 kg kotoran sapi + 500 ml air + 0,25 kg Cacing, V3 : 0,5 kg serbuk gergaji + 0,5 kg kotoran sapi + 500 ml air + 0,25 kg Cacing + 10 ml EM4, V4 : 0,5 kg serbuk gergaji + 0,5 kg kotoran sapi + 500 ml air + 0,25 kg Cacing + 15 ml EM4. Hasil dari penelitian ini adalah Variasi perlakuan kompos yang paling efektif adalah perlakuan V2 dengan penambahan cacing dengan nilai C/N rasio, pH, suhu, dan warna sudah sesuai dengan standar SNI 19-7030-2004. Penambahan cacing dan dekomposer EM4 berpengaruh terhadap perubahan suhu, pH, warna dan C/N Rasio, hal ini dibuktikan dari hasil pengujian yang berbeda-beda pada setiap perlakuan.
Toxic and Hazardous (B3) Solid Waste Management at Abdul Moeloek General Hospital: An Implementation Assessment in 2022 and Recommendations Hasiany, Sillak; Naibaho, Riadi Tomson Eventius; Lisafitri, Yuni; Putri, Intan Andriani
Indonesian Journal of Environmental Management and Sustainability Vol. 7 No. 1 (2023): March
Publisher : Magister Program of Material Science, Graduate School of Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26554/ijems.2023.7.1.32-37

Abstract

Making sure that the health and environment are taken care of, solid B3 Waste produced by hospitals has to be treated according to existing regulations. As an A-class hospital in Bandar Lampung, AM General Hospital commit to conducting good practices for its solid B3 waste management. This study aims to analyze the current implementation of solid B3 waste management, the compliance of AM General Hospital’s B3 waste management Standard Operation Procedure (SOP) with current regulations, and recommend improvement strategies for solid B3 waste management in AM General Hospital. For eight consecutive days in August 2022, solid B3 waste samples were collected and analyzed. The average solid B3 waste produced by AM General Hospital was 285 kg/day for infectious waste and 6.3 kg/day for sharp waste. Inpatient installation contributed the majority of solid B3 waste there. Due to the observation sheet that was created according to the regulations, 65% of AM General Hospital’s SOP involving sorting; storage; collection; transportation; and disposal has already complied with the regulations. Several shortcomings were found such as the absence of an official solid B3 waste handling report; the absence of proper labeling in the B3 waste containers; and the use of black plastic bags. Analytical Hierarchy Process (AHP) data analysis technique was deployed, resulting in a technology application and waste minimization as improvement recommendations.
Analisis Variasi Jenis Perekat Tetes Tebu dan Tepung Tapioka Pada Pemanfaatan Limbah Ampas Tebu Menjadi Briket Mufti, Aulia Annas; Akram, Muhammad; Lisafitri, Yuni; Kurnianingtyas, Erlina
Al-Ard: Jurnal Teknik Lingkungan Vol. 9 No. 2 (2024): March
Publisher : Department of Environmental engineering, Faculty of Science and Technology, Islamic State University Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/alard.v9i2.1857

Abstract

Energy is a factor that has a very important role in various economic activities and people's lives. The availability of energy and fuel oil is currently lacking, indicating the need to switch to alternative fuels, one of which is charcoal briquettes. Sugarcane bagasse is one of the many biomasses that can be used. as a raw material for making briquettes. The process of making charcoal briquettes requires additional material in the form of an adhesive whose purpose is to glue the charcoal powder so that it becomes a solid form. As a binder for briquettes, molasses has the potential to increase the calorific value because it is rich in carbon content derived from sucrose which is abundant in molasses. Therefore, a study was conducted to analyze the fuel characteristics of bagasse briquettes with adhesive variations in the form of tapioca flour and molasses. The parameters to be tested are moisture content, ash content, volatile matter content during the heating process, bound carbon and heating value. Research on the production of charcoal briquettes was carried out using bagasse waste with variations in the addition of tapioca adhesive and molasses. It can be concluded that the best quality briquettes are A1 briquettes, namely bagasse briquettes with 0.5 gram of adhesive drops, which are close to the standard criteria of briquettes, namely the highest calorific value, lowest moisture content, lowest ash content and lowest volatile matter content.