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All Journal Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology
Ambrose Louise
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology

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Anticancer Potential of Three Sea Cucumber Species Extracts on Human Breast Cancer Cell Line Rameshkumar Santhanam; Nurul Shahirah Mohd Azam; Ammira Shafiqha Abdul Khadar; Ambrose Louise; Gregory Dominic; Nur Shahida Ahmad Sofian; See Wee Han; Thiruventhan Karunakaran; Thilahgavani Nagappan; Tengku Sifzizul Tengku Muhammad3; Sevakumaran Vigneswari
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 17, No 2 (2022): August 2022
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.669

Abstract

Sea cucumber has long been utilized as a treatment for a variety of ailments, including antibacterial, antifungal, immunomodulatory, and wound healing. As for the first time, the extracts of three sea cucumber species’ i.e, Actinopyga lecanora, Holothuria atra, and Stichopus vastus, were compared and tested on the cytotoxicity of cancer cells using MTT and Annexin V/Propidium Iodide assays. This study investigates the protection of sea cucumber extracts against the breast cancer cell line (T-47D). All three extracts were found to be actively declining the cancer cell progression, with the IC50 values of 6.25±0.50 µg/ml (A. lecanora), 7.5 ± 1.39 µg/ml (H. atra) and 3.25 ± 0.53 µg/ml (S. vastus). LC-MS/MS analysis was used to identify chemical compunds in the extracts. The 1,1-diphenyl-2-picrylhydrazyl (DPPH), total phenolic and flavonoid contents, and anti-collagenase activity were also assessed in all the three extracts. The results demonstrated the absence of antioxidant and flavonoid chemicals in S. vastus, A. leconara and H. atra extracts. However, H. atra contained phenolic compounds (0.4794 mgGAE/g DW). Furthermore, all tested extracts showed significant anti-collagenase activity, which supported the reduction of cancer cell proliferation. However, more research into the mechanism of action of the extract is needed before sea cucumbers therapeutic characteristics may be used to combat breast cancer.
Co-Authors Ammira Shafiqha Abdul Khadar Gregory Dominic Nur Shahida Ahmad Sofian Nurul Shahirah Mohd Azam Rameshkumar Santhanam See Wee Han Sevakumaran Vigneswari Tengku Sifzizul Tengku Muhammad3 Thilahgavani Nagappan Thiruventhan Karunakaran