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All Journal Asian Journal of Health Research
Suheni Ninik Hariyati
Department of Obstetrics and Gynaecology, Faculty of Medicine, Universitas Brawijaya/ Saiful Anwar General Hospital, Malang, East Java, Indonesia
Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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Effect of Escherichia Coli on Decorin and Type I Collagen Levels in Fetal Membranes of Premature Balb/c Mice Nirwan Nugraha; Suheni Ninik Hariyati; Didik Agus Gunawan; Mokhammad Nooryanto; Pande Made Dwijayasa
Asian Journal of Health Research Vol. 1 No. 3 (2022): Volume 1 No 3 (December) 2022
Publisher : Ikatan Dokter Indonesia Wilayah Jawa Timur

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55561/ajhr.v1i3.26

Abstract

Introduction: Premature birth is a global problem in various countries worldwide. The pathogenesis leading to preterm labor may occur in early pregnancy and is associated with inflammatory pathways and changes in the angiogenic process. Decorin and biglycan are hypothesized to play a role in pregnancy maintenance, wherein preterm delivery, these levels are thought to be reduced. This study was examined effect of Escherichia coli on Decorin and Type I Collagen levels in fetal membranes of Premature Balb/c Mice Methods: There were 28 pregnant Balb/c mice divided into 4 groups, with a post-test-only control group design in true experimental design research. The first control group (N1) was a group of pregnant mice dissected on day-15, while the second control group (N2) were mice pregnant at term followed up to parturition. Group P1 were given Escherichia coli 1x109 CFU/ ml dose on the cervix on day-15, while the second treatment group (P2) were given Escherichia coli in the cervical canal on day-15 followed by delivery. We examined the Decorin and Type I Collagen levels between group. One-way ANOVA was used to analyze the collected data. Results: There was a significant difference in the mean decorin levels between the control group N1 vs P2 (12.35±2.24 ng/mL vs 6.62±1.50 ng/mL; p=0.000). In addition, a significant difference was also found in the mean level of type 1 collagen between the control group N1 vs P2 group (283.5±31.3 ng/mL vs 170.6±38.8 ng/mL; p=0.000). Conclusion: A decrease in decorin levels affects the reduction in collagen type 1 levels in fetal membranes of Balb/C mice in premature models.
The Effect of Exposure to Escherichia coli with Low Molecular Weight Hyaluronan, High Molecular Weight Hyaluronan, Hyaluronidase in the Cervix Premature Pregnant Model of Balb/C Mice Fikhy Rizky Hapsari; Suheni Ninik Hariyati; I Wayan Arsana Wiyasa; Teguh Wiyono; Dewi Santosaningsih
Asian Journal of Health Research Vol. 1 No. 3 (2022): Volume 1 No 3 (December) 2022
Publisher : Ikatan Dokter Indonesia Wilayah Jawa Timur

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55561/ajhr.v1i3.56

Abstract

Introduction: Infection is the biggest cause of preterm labor, about 25-40%, and ascending infection through the vagina is the most common route of infection. This study aims to evaluate the level of Low Molecular Weight Hyaluronan (LMWHA), level of High Molecular Weight Hyaluronan (HMWHA), and the level of the enzyme hyaluronidase in Balb/c premature labor due to infection with Escherichia coli model mice. Methods: This study used an actual experimental design carried out in the laboratory in vivo on balb/c mice exposure to Escherichia coli with a randomized post-test only with a control group design. In the premature model of exposure to Escherichia coli balb/c mice on the cervix of 109 CFU/ml, cervical samples were taken and the levels of LMWHA, HMWHA, and hyaluronidase were measured using spectrophotometry. This study was divided into 2 groups. Control group and treatment group. Results: Low Molecular Weight Hyaluronan (LMWHA) and of the hyaluronidase enzyme levels in premature Balb/C cervical mice exposed to E. coli were higher than in Balb/C cervical mice. High Molecular Weight Hyaluronan (HMWHA) levels in premature Balb/C cervical mice exposed to E. coli were lower Conclusion: In the cervix of the infected by E. coli mice model, LMWHA and hyaluronidase enzymes increased and HMWHA decreased.    
The Effect of Establishing Escherichia coli on Balb/C Rats as A Model of Preterm Labor Suheni Ninik Hariyati; Dewi Santosaningsih; Arief Satria Hardika; Fikhy Rizky Hapsari; Nirwan Nugraha L.; Wildan Aulia Firdaus
Asian Journal of Health Research Vol. 2 No. 2 (2023): Volume 2 No 2 (August) 2023
Publisher : Ikatan Dokter Indonesia Wilayah Jawa Timur

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55561/ajhr.v2i2.115

Abstract

Introduction: The prevalence of preterm labor in Indonesia is estimated at 7-14%, which is around 459,200-900,000 babies per year. The mechanism of infection is the biggest cause of preterm labor. The bacteria that often causes infection is Escherichia coli. This study aims to see the effect of giving Escherichia coli to produce premature models of mice. Material and Methods: This study used a true experimental method with a post-test only control group design approach. The independent variables were preterm labor & cervical tissue culture, amniotic membranes, and placenta. The dependent variable of the study was the administration of Escherichia coli 109 CFU to the cervix. This research will be tested with a comparative test. Results: The treatment group were given Escherichia coli experienced parturition with the fastest time of 38 hours 22 minutes and the longest time of 42 hours 12 minutes. Escherichia coli colonies on Eosin Methylene Blue (EMB) culture media with a glowing green image was obtained. Cervical culture, amniotic membranes and placenta from the treatment group of Balb/C mice showed the growth of Escherichia coli colonies. Neutrophil infiltration and edema in the cervical stroma was found to be more severe in Balb/C mice that received treatment with Escherichia coli 109 CFU/ml. Conclusion: Escherichia coli shortens the time of delivery and increases the bacterial load in pregnant Balb/C mice.
Co-Authors Arief Satria Hardika Dewi Santosaningsih Didik Agus Gunawan Fikhy Rizky Hapsari Mokhammad Nooryanto Nirwan Nugraha Nirwan Nugraha L. Pande Made Dwijayasa Teguh Wiyono Wildan Aulia Firdaus Wiyasa, I Wayan Arsana