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All Journal Indonesian Journal of Chemistry Pharmaceutical And Biomedical Sciences Journal (PBSJ)
Isra Janatiningrum
UIN Syarif Hidayatullah Jakarta, South Tangerang 15419, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Health Professions Medicine & Pharmacology Public Health

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Isolasi dan Karakterisasi Bakteri Endofit dari Daun Sirsak Annona Muricata L. dan Aktivitas Antibakterinya terhadap Beberapa Bakteri Patogen Isra Janatiningrum; Akhmad Endang Zainal Hasan; Eny Ida Riyanti
Pharmaceutical and Biomedical Sciences Journal (PBSJ) Vol 3, No 2 (2021)
Publisher : UIN Syarif Hidayatullah Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/pbsj.v3i2.24321

Abstract

Soursop (Annona muricata L.) is a plant with many health benefits. Soursop leaves are widely used as traditional medicine by Indonesian citizen for wound infection healing. The antibacterial activity of soursop leaves cannot be separated from the presence of endophytic microbes that live inside. Endophytic bacteria live in plant tissues and have beneficial for their host plants. Endophytic microbes are able to produce same secondary metabolites as their host such as producing antibacterial compounds. Thirty one bacterial endophytes were isolated from soursop leaves that from 3 different regions: Garut, Cianjur, and Sukabumi. Morphologically, endophytic bacterial isolates from the three regions had different diversity. Bacterial isolates were tested for their activity against 4 test pathogenic bacteria, such as Pseudomonas aeroginosa, Escherichia coli, Staphylococcus mutans, and Streptococcus aureus. Antibacterial test using disc diffusion method with chloramphenicol 500 m/mL as positive control. Based on the antibacterial activity test, isolate G42 was able to inhibit all types of test bacteria. G42 isolates forms a zone of inhibition up to 17 mm in P. aeroginosa. Based on 16S rRNA, G42 isolates had similarity with Pantoea wallisii up to 93.24%. This study shows that endophytic bacteria isolated from soursop leaves have the potential to be developed into antibacterial agents.
Characterization of α-Glucosidase Inhibitor Streptomyces sp. IPBCC.a.29.1556 Aqueous Extract: An Endophyte of Indonesian Ficus deltoidea Isra Janatiningrum; Yulin Lestari; Dedy Duryadi Solihin; Anja Meryandini
Indonesian Journal of Chemistry Vol 22, No 6 (2022)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.72433

Abstract

Filamentous bacteria have been known as actinobacteria which could produce various secondary metabolites, including an α-glucosidase inhibitor. The α-glucosidase inhibitor has been identified to be potentially valuable for the treatment of diabetes mellitus. Endophytic actinobacteria are able to produce bioactive compounds that are similar to their hosts. Indonesian Ficus deltoidea is one of the medicinal plants which has the activity of the α-glucosidase inhibitor. The α-glucosidase inhibitor has been characterized by optimizing compound production, fractionation, analysis using TLC and LC-MS, and identifying inhibitor mechanisms. The α-glucosidase inhibitor substance is present in Streptomyces sp. IPBCC.a.29.1556 aqueous extract. The aqueous extract was separated and fraction 1 had an IC50 value of 58.8 μg/mL, which is better than acarbose (IC50 = 90.4 μg/mL). Kinetic studies revealed that this fraction inhibited the enzyme through a non-competitive mechanism. Chemical profile based on LC-MS, fraction 1 showed the presence of Phenylpropynal, Butyric acid, 2-(2-Ethoxyethoxy)ethanolate, 1,1-Diethoxyethane acetate, N,N-dimethyl-3-oxide-1H-Benzotriazole-1-propanamine, p-coumaric acid, and isoquinolinium which might contribute individually or synergistically to the observed α-glucosidase inhibitor activity. These results suggest that fraction 1 from the aqueous extract of Streptomyces sp. IPBCC.a.29.1556 is the potential source to produce an α-glucosidase inhibitor for the management of postprandial hyperglycemia.
Co-Authors Akhmad Endang Zainal Hasan Anja Meryandini Dedy Duryadi Solihin Eny Ida Riyanti YULIN LESTARI