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Yohanes Alvin Goenawan
Fakultas Teknobiologi, Universitas Surabaya, Surabaya
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Economics, Econometrics & Finance Social Sciences

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LYCOPENE CONTENT IN SEVERAL AGES OF TOMATO CALLUS (Lycopersiconesculentum Mill. cv. Rampai) Yohanes Alvin Goenawan; Katherine Krisyanti Khodjojo; Popy Hartatie Hardjo; Maria Goretti Marianti Purwanto
Agric Vol. 34 No. 1 (2022)
Publisher : Fakultas Pertanian dan Bisnis, Universitas Kristen Satya Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24246/agric.2022.v34.i1.p89-104

Abstract

Tomato (Lycopersiconesculentum Mill. cv. Rampai) is rich in lycopene, a carotenoid group compound with many biological activities, one of which is as an antioxidant. Conventional tomato cultivation is not optimal due to the susceptibility of tomato plants to pests and diseases, even though the need for lycopene continues to increase. Plant tissue culture method can be one solution for lycopene production. Callus tomato cv. Rampai was initiated from cotyledons on MS medium (Murashige& Skoog) with the addition of 1 ppm BA (benzyladenine) and 1.5 ppm IAA (indole-3-acetic acid). Callus aged one week to four weeks were harvested and lycopene was extracted by maceration method using hexane:acetone (9:1) as solvent. The presence of lycopene in callus was confirmed by TLC (thin-layer chromatography) (retention factor value/Rf = 0.56) and UV-Vis spectrophotometry (wavelength that gives maximum absorbance = 472 nm). Lycopene content in tomato callus increased with increasing callus age and was directly proportional to the callus growth index. The highest lycopene level was found in callus aged 4 weeks, which was 0.3094 mg/100 g dry weight callus.
Co-Authors Katherine Krisyanti Khodjojo Maria Goretti Marianti Purwanto Popy Hartatie Hardjo