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All Journal Jurnal Biologi Tropis
Vannesa El Shaday Ruth Advenita
Universitas Pelita Harapan
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

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Isolation and characterization of indigenous copper resistant bacteria from Yogyakarta tannery factory waste Wahyu Irawati; Polin Parulian Ambarita; Desi L. Sihombing; Vannesa El Shaday Ruth Advenita; Eunike Bunga Marvella
Jurnal Biologi Tropis Vol. 22 No. 3 (2022): July - September
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v22i3.3621

Abstract

The leather tanning industry is one of the industries that causes environmental damage because one of the processes uses copper to produce products such as bags, jackets, and shoes. Copper bioremediation using indigenous bacteria is an effective solution to the problem of pollution because it utilizes bacteria isolated from the polluted environment. This study aims to isolate and characterize copper-resistant bacteria from the waste of the Yogyakarta Tannery Industry. The medium used was Luria Bertani Agar with the addition of 3 mM CuSO4. The characterization carried out included the characterization of colony and cell morphology such as color, edges, optical appearance, cell shape, and Gram staining. The isolation results obtained eight isolates of copper-resistant bacteria, namely Y1, Y2, Y3, Y4, Y5, Y6, Y7, and Y8. Isolates Y1, Y2, Y3, Y4, Y5, and Y8 were Gram positive bacteria while isolates Y5 and Y6 were Gram negative bacteria. Colonies of isolates Y1, Y3, Y4, Y8 greenish color indicates the accumulation of copper in the cells as a mechanism of resistance. The discovery of isolates of copper-resistant bacteria from samples of the tannery industry waste is a useful study as an initial study to obtain isolates of Indonesian indigenous copper-resistant bacteria to be further developed as a copper bioremediation agent in the treatment of copper waste in the leather tanning industry.
The Potency of Vitamin C in Tomato Plant for the Result of Genetically Modified Lanceolate Gene Through Agrobacterium Tumefaciens Using CRISPR-CAS 9 Vannesa El Shaday Ruth Advenita; Christeven Mevotema; Iren Asima Situmorang; Lusiana Haris; Wahyu Irawati
Jurnal Biologi Tropis Vol. 23 No. 1 (2023): January - March
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i1.4682

Abstract

Vitamin C is the important part in the formation of protein in the body, one of which is obtained from tomatoes (Solanum lycopersicum L.). Due to the needed of vitamin C, a way is needed to get vitamin C from tomatoes which are of higher value. Tomato genetically modified using the lanceolate gene through Agrobacterium tumefaciens using CRISPR-CAS9 is a promising solution for the food world. The literature study method is carried out by reviewing the theory and the results of previous research qualitatively. The results is that genetically engineered tomatoes have the advantage of high vitamin content by using plant improvement and modification. The bacterium Agrobacterium tumefaciens functions as a vector and insertion site for recombinant genes and CRISPR-CAS9 technology is used to modify the target genome because it has a CAS9 protein containing two homologous domains resulting in a new trait. Tomatoes with lanceolate leaves will have fruit with high vitamin content because the results of photosynthesis are focused on fruit development. Suggestions for conducting in-depth research to ensure safety in the food quality and health. The goals of the research were to 1) determine the potency of tomato rich in vitamin C using genetic engineering of Agrobacterium tumefaciens, 2) structure and function of the Lanceolate gene in plants, 3) application of CRISPR-CAS 9 in genetic engineering, and 4) advantages and disadvantages of genetically modified tomato. God gave humans the ability to manage His creation. So that humans can glorifying God through research.
Co-Authors Christeven Mevotema Desi L. Sihombing Eunike Bunga Marvella Iren Asima Situmorang Lusiana Haris Polin Parulian Ambarita WAHYU IRAWATI