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Isolation, Identification and Antibacterial Testing, Indigenous Bacteria from Apis Mellifera's Honeycomb North Sumatra Origin Esibrena Br Kemit; Yermia S. Mokosuli; Helen J Lawalata
Jurnal Biologi Tropis Vol. 22 No. 3 (2022): July - September
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v22i3.4343

Abstract

Honeycomb can be used as a source of antibacterial, this is due to the content of secondary metabolites in the form of flavonoids in the honeycomb which serves as a protective determinant of honey quality. This study aims to isolate, identify and test antibacterial isolates of Indigenous bacteria from Apis mellifera nests against gram-positive bacteria S. aureus and gram-negative bacteria E. coli. This research used descriptive method and the research data were obtained through laboratory experiments. The results of the isolation stage of indigenous bacteria obtained seven (7) isolates AM1, AM2, AM3, AM4, AM5, AM6, AM7 Indigenous bacteria from Apis mellifera bee hives. AM1 and AM4 isolates were similar to the Enterobacter genus, AM2 isolates to Corynebacterium genus, AM3 and AM6 isolates to Paracoccus genus, and AM5 and AM7 isolates to Azotobacter Sp. The seven isolates of Indigenous bacteria have potential as antibacterial and the diameter of the inhibition zone against E. coli bacteria is AM1 (8.88 mm), AM2 (8.65 mm), AM3 (8.03 mm), AM4 (6.41 mm) , AM5 (9.07 mm), AM6 (9.53 mm) and AM7 (9.44 mm) while against S. aureus AM1 (9.08 mm), AM2 (9.23 mm), AM3 (9.15 mm), AM4 (8.70 mm), AM5 (10.44 mm), AM6 (11.56 mm) and AM7 (9.0 mm).
STUDY OF MANGROVE VEGETATION COMMUNITY AT BUNAKEN NATIONAL PARK AREA, TELING VILLAGE Febiola Gabriela Tular; Helen J Lawalata; Marthy L. S. Taulu
Indonesian Biodiversity Journal Vol. 3 No. 3 (2022): December 2022
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Mangrove vegetation generally grows to form a zoning starting from a beach to a view of meters inland. Mangrove zoning consists of Avicennia plant species that face directly toward the sea and behind or on the edge of the Avicennia plant. There is the Riophora plant. Towards the mainland, there are Bruguiera plants and, finally, the Ceriops plant which grows and interacts with small bushes. Each type of Mangrove plant has a level; the first is the seedling level, the second is the sapling level, the third is the pole level, and the fourth is the tree level. This study aims to analyze the Mangrove Vegetation Community in Bunaken Nation Park, Teling Village, using a descriptive survey method and constructing three transects, which are transected measuring 10×10 m2, 5×5 m2, and 2×2 m2. The result on each transect shows that in Bunaken National Park, Teling Village, there were types of Mangrove Vegetation Communities, namely Avicennia plant species with a total of 129, Rhizophora plant species with a capacity of 122 and Bruguiera plant species with a total of 91, where each plant type it has levels with different numbers.
STUDY OF MANGROVE VEGETATION COMMUNITY AT BUNAKEN NATIONAL PARK AREA, TELING VILLAGE Febiola Gabriela Tular; Helen J Lawalata; Marthy L. S. Taulu
Indonesian Biodiversity Journal Vol. 3 No. 3 (2022): December 2022
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v3i3.5592

Abstract

Mangrove vegetation generally grows to form a zoning starting from a beach to a view of meters inland. Mangrove zoning consists of Avicennia plant species that face directly toward the sea and behind or on the edge of the Avicennia plant. There is the Riophora plant. Towards the mainland, there are Bruguiera plants and, finally, the Ceriops plant which grows and interacts with small bushes. Each type of Mangrove plant has a level; the first is the seedling level, the second is the sapling level, the third is the pole level, and the fourth is the tree level. This study aims to analyze the Mangrove Vegetation Community in Bunaken Nation Park, Teling Village, using a descriptive survey method and constructing three transects, which are transected measuring 10×10 m2, 5×5 m2, and 2×2 m2. The result on each transect shows that in Bunaken National Park, Teling Village, there were types of Mangrove Vegetation Communities, namely Avicennia plant species with a total of 129, Rhizophora plant species with a capacity of 122 and Bruguiera plant species with a total of 91, where each plant type it has levels with different numbers.
IDENTIFICATION OF THE TYPE OF FERNS IN THE FOREST AREA OF NOONGAN VILLAGE Yosua Linu; Helen J Lawalata; Rievo Djarang
Indonesian Biodiversity Journal Vol. 4 No. 1 (2023): April 2023
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v4i1.5782

Abstract

Ferns (Pteridophyta) are spore-forming cormophytes that can live in a variety of habitats both as epiphytes, terrestrial and aquatic. In the forest area of Noongan Village, many potentials play an important role in developing forest ecosystems. One such potential is ferns. The results of the study were eight types of ferns in the forest area of Noongan village which were divided into two plots of 50x50 meters at different heights, namely plot I at an altitude of 610 masl with a total of 380 ferns with the percentage: Paku Garuda (Pteridium aquilinium) 0.20 %, Elephant fern (Angiopetris avecta Hoofm) 0.01%, Red rane fern (Selaginella australis) 0.01%, Paku Kikir (Stenosomia Sp.) 0.14%, Ferret fern (Nephrolepis biserrataSchott.) 0, 21%, cecerenean fern (Nephrolepis falcata) 0.16%, suplir fern (Adiantum.) 0.12%, andam fern (Dicranopetris linearis) 0.12%. And plot II at an altitude of 869 meters above sea level with a total of 179 ferns with a percentage of: Garuda fern (Pteridium aquilinium) 0.21%, elephant fern (Angiopetris avectaHoofm) 0.01%, miserly fern (Stenosomia Sp.) 0, 17%, Paku harupat (Nephrolepis biserrate Schott.) 0.21%, Paku cecerenean (Nephrolepis falcata) 0.15%, Suplir (Adiantum.) 0.10%, Paku andam (Dicranopetris linearis) 0.12%. In the plot I, the types and number of ferns were higher than the types and numbers of ferns in plot II. No red fern (Selaginella australis) was found in plot II. And the plant species Paku Harupat (Nephrolepis biserrata Schott). The red fern has the highest number, and the red fern (Selaginella australis.) has the least amount. The difference in altitude greatly affects the surrounding environment. So that these differences greatly affect the number of types and numbers of ferns
Analisis Residu Pestisida Pada Tanaman Kentang (Solanum tuberosum L) di Kecamatan Modoinding Kabupaten Minahasa Selatan Bricita Regina Warangkiran; Orbanus Naharia; Helen J Lawalata
J-CEKI : Jurnal Cendekia Ilmiah Vol. 3 No. 4: Juni 2024
Publisher : CV. ULIL ALBAB CORP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56799/jceki.v3i4.3685

Abstract

Tujuan penelitian ini adalah untuk mengetahui adanya residu pestisida pada kentang yang dibudidayakan warga Kecamatan Modoinding Kabupaten Minahasa Selatan. Penelitian ini bercirikan penelitian kualitatif yang dilakukan dengan pendekatan eksperimen laboratorium. Penelitian ini akan dilakukan di Laboratorium Pengujian dan Perlindungan Mutu Tanaman dan Hortikultura Kalasey pada bulan September hingga Oktober 2023. Tujuan penelitian ini adalah untuk memastikan adanya residu pestisida pada tanaman kentang yang dibudidayakan di perkebunan masyarakat yang terletak di Desa Sinisir, Desa Sinsingon, dan Desa Wulurmaatus. Sampel yang digunakan terdiri dari sembilan sampel yang diperoleh dari buah-buahan, dengan tiga ulangan untuk setiap sampel. Temuan penelitian residu pestisida pada tanaman kentang di Kecamatan Modoinding Kabupaten Minahasa Selatan tahun 2023 dapat diringkas sebagai berikut: Tidak ditemukan jejak pestisida pada tanaman kentang di Desa Wulurmaatas Kecamatan Modoinding Kabupaten Minahasa Selatan. Tanaman kentang di Desa Sinisir, Kecamatan Modoinding, Kabupaten Minahasa Selatan bebas dari pestisida apa pun. Tanaman kentang di Desa Sinsingon, Kecamatan Modoinding, Kabupaten Minahasa Selatan bebas dari pestisida apa pun. Tanaman kentang di Kecamatan Modoinding Kabupaten Minahasa Selatan yaitu di Desa Wulurmaatas, Desa Sinisir, dan Desa Sinsingon bebas residu pestisida sehingga aman dikonsumsi.