Rinaldi B. Utomo
Department of Pediatric Dentistry, Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta, Indonesia

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The effect of green robust coffee gel (coffea canephora) to collagen density on gingival wound healing (an in-vivo study in wistar rats) Ni-Putu I. Suryadewi; Rinaldi B. Utomo; Indra Bramanti
Journal of Dentomaxillofacial Science Article In Press
Publisher : DiscoverSys Inc.

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Abstract

Objective: This study aims to determine the effect of robusta green coffee gel on the collagen density in Wistar rats. Material and Methods: The subjects were 45 Wistar rats, each wounded with a 3 mm punch biopsy in the mandibular incisor gingiva. The rats were then divided into 5 groups, namely the positive control group (Povidone-iodine 10%), negative control (gel base), and 3 treatment groups (10%, 20%, and 40% concentration of Robusta green coffee gel). The intervention was carried out twice a day. A total of 3 mice in each group were then sacrificed on the 5th and 10th days to see the collagen density using the Mallory Trichrome staining technique. Collagen density was observed using a microscope with 400x magnification in 5 fields of view. Kruskal Wallis test was used for data processing. Results: There was no difference in collagen density regardless of the coffee gel concentration at each observation time, with p>0.05. Conclusion: There was no effect on the times of observation or dose concentration of robusta coffee gel on collagen density on gingival wound healing in Wistar rats.