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All Journal Bioscientist : Jurnal Ilmiah Biologi
Erlin Yustin Tatontos
Jurusan Teknologi Laboratorium Medis, Poltekkes Kemenkes Mataram
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Social Sciences

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Potensi Tepung Biji Kecipir (Psophocarpus tetragonolobus) sebagai Bahan Alternatif Sumber Nitrogen dalam Media Mannitol Salt Agar (MSA) untuk Pertumbuhan Staphylococcus aureus Urip Urip; Nanda Yuniza Pratiwi; Erlin Yustin Tatontos; Maruni Wiwin Diarti
Bioscientist : Jurnal Ilmiah Biologi Vol 10, No 2 (2022): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v10i2.6138

Abstract

The purpose of this study was to determine the potential of winged bean (Psophocarpus tetragonolobus) seed powder as an alternative medium for Mannitol Salt Agar (MSA) against the growth of Staphylococcus aureus bacteria. This research method is true experiment with Completely Randomized Design (CRD). The experimental unit of this research was winged bean seed flour and Staphylococcus aureus. Winged bean seed flour used with 5 treatments, namely 2 gr, 4 gr, 6 gr, 8 gr, and 10 gr with each replication 5 times. The results showed that the standard MSA as a control showed an average of 3211 colonies, the average colony at a concentration of 2% was 372 colonies, the average colony on a 4% concentration plate was 419 colonies, the average colony on a 6% concentration plate was 544 colonies, the average colony on an 8% concentration plate were 622 colonies, and the average colony on a 10% concentration plate was 816 colonies. The conclusion of this study is winged bean seed powder has the potential as an alternative source of nitrogen in Mannitol Salt Agar (MSA) media for the growth of Staphylococcus aureus.
Co-Authors Maruni Wiwin Diarti Nanda Yuniza Pratiwi Urip Urip