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All Journal Interdental Jurnal Kedokteran Gigi (IJKG) Journal of Health and Dental Sciences Acta Medical and Health Sciences (AMHS)
Euis Reni Yuslianti
Biochemistry Department, Faculty Of Dental Medicine, Universitas Jenderal Achmad Yani, Cimahi, Jawa Barat, Indonesia
Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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Development of a novel diagnostic kit candidate to detect dengue antibody, using IgY and protein a positive Staphylococcus aureus Eka Noneng Nawangsih; Lia Siti Halimah; Euis Reni Yuslianti; Sayu Putu Yuni Paryati
ACTA Medical Health Sciences Vol. 1 No. 3 (2023): ACTA Medical Health Sciences
Publisher : ACTA Medical Health Sciences

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Abstract

Early diagnosis of dengue hemorrhagic fever (DHF) is very important in determining further management. Diagnostic kits are intended as relatively faster, easier, and cheaper diagnostic tools. The objectives of this experimental laboratory study was to prepare a diagnostic kit candidate to detect dengue antibody in human serum using co -agglutination methods by utilizing protein A positive Staphylococcus aureus as a carrier to bind Fc immunoglobulin fractions without altering its ability to bind antigen. To enhance the sensitivity and specificity of the diagnostic kit, chicken IgY was used as one of the components. Rabbit immunoglobulin chicken anti-IgY was used as an intermediary connection between protein A positive and Staphylococcus aureus with chicken IgY. Solution A contains Staphylococcus aureus Cowan I and rabbit immunoglobulin chicken anti-IgY serum while Solution B contains anti-dengue chicken serum and dengue antigen. The laboratory experiments produced a formula of A: B = 1~3: 1~3 (v/v), which was then tested to human serum and compared with IgM and IgG ELISA tests as the gold standard. Data on the results of the test were statistically analyzed using chi - square test. Of the 65 qualified samples used, 47.69% resulted in true positive and 41.54% resulted in true negative. Meanwhile, the remaining samples demonstrated false negative (6.15%), positive predictive value (91.2%) and negative predictive value (87.1%). The sensitivity and specificity were 89% and 90%, respectively, with no significant diffe rences (p>0.05). Therefore, co-agglutination method using S. aureus with protein A positive as a carrier can be proposed as a diagnostic kit candidate to detect human serum dengue antibody to screen dengue hemorrhagic fever cases.   DOI : 10.35990/amhs.v1n3.p112-120
Potential Of Keprok Citrus (Citrus nobilis) From Lembang West Java For Dental Email Cleaning Euis Reni Yuslianti; Hartanto Endrowahyudi; Asih Rahaju; Orin Oktarina
Interdental Jurnal Kedokteran Gigi (IJKG) Vol. 20 No. 2 (2024): Interdental Jurnal Kedokteran Gigi (IJKG)
Publisher : Fakultas Kedokteran Gigi, Universitas Mahasaraswati Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46862/interdental.v20i2.7092

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Introduction: Consumption of colored foods and drinks can cause tooth discoloration, synthetic whitening agents are used to increase the degree of brightness of tooth color but have many side effects. Tangerine (Citrus nobilis) is a natural ingredient that is used by the community for whitening the color of teeth safely and with minimal side effects. This study aims to determine the potential of the ethanol extract of tangerine peels to whiten teeth in terms of the degree of color brightness and tooth enamel surface roughness. Material and Methods: This research analytic laboratory experiments. Samples used 30 premolars that had been soaked in coffee, divided according to the concentration of tangerine peel extract 25%, 50%, and 75% then each group was exposed to 1x30 and 2x30 minutes. Tooth discoloration seen from the value of the degree of color brightness and tooth enamel surface roughness before and after exposure was analyzed using the paired T test and Univariate GLM test (P<0.05).  Results and Discussions: The result showed there were differences in the effect of ethanol extract of tangerine peel on the brightness of the color and roughness of tooth enamel at various concentrations before and after treatment and there was an interaction effect between concentrations of 25%, 50%, and 75% and exposure time of 1x30 and 2x30 minutes which was significant with p=0 .00. Conclusion: It can be concluded that ethanol extract of tangerine peel has the potential to whiten teeth as seen from the degree of color brightness and tooth enamel surface roughness.
MORSICATIO BUCCARUM AND LABIORUM IN SEVERE ANXIETY PATIENT Sutrania Dewi Sulaksana; Euis Reni Yuslianti
Journal of Health and Dental Sciences Vol. 4 No. 2 (2024): Journal of Health and Dental Sciences
Publisher : Fakultas Kedokteran Gigi Unjani

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Abstract

Morsicatio buccarum and labiorum are conditions generally caused by chronic habitual cheek and lip biting. Objective findings present thick, shaggy, irregular white lesions on the buccal and labial mucosa. Morsicatio is frequently associated with psychological conditions. This case report aims to assess the depression, anxiety, and stress levels in a patient with morsicatio buccarum and labiorum. A 22-year-old male patient visited the Integrated Polyclinic of Universitas Jenderal Achmad Yani Dental Hospital to check his oral status. Clinical examination revealed incidental findings. Extraoral examination showed clicking on both temporomandibular joint (TMJ) and lip desquamation sides. Intraoral mucosa examination revealed white lesions in the form of unsweepable plaque, irregular, slightly elevated from surrounding tissue, and interspersed with erythematous erosion areas on the buccal and labial mucosa. A pigmented lesion was found on the anterior attached gingiva mucosa on both the upper and lower jaws. The patient admitted to chronic habitual cheek biting since childhood. The Depression, Anxiety, and Stress-21 (DASS-21) assessment showed anxiety reaching a severe level. A psychological approach is needed as part of the treatment planning in morsicatio cases. DOI : 10.54052/jhds.v4n2.p147-156
Co-Authors Asih Rahaju Eka Noneng Nawangsih Hartanto Endrowahyudi Lia Siti Halimah Orin Oktarina Sayu Putu Yuni Paryati Sutrania Dewi Sulaksana