The objective of the research was to know of the responses of the aplication Rhizobium of nodule root Soybean as well as nodule root Mimosa pudica and Trichoderma iso- late Tc-Jjr02. The research was conducted on the Green House Faculty of Agriculture, Muhammadiyah University of Sidoarjo, from Januari to Mei 2016. This research com- piled using a factorial randomized complete design which consists of 2 factors : factor I is application Rhizobium consisting of : R0 (withow Rhizobium), R1(Rhizobium of soy- bean), R2(Rhizobium of mimosa pudica). Factor II application Trichoderma consisting of : T0 (withow Trichoderma) and T1 (Trichoderma isolat Jati Jejer-02). Of these two factors obtained 6 treatment combinations and repeated 4 times. From the analysis of statisti- cal data shows that application Rhizobium on significant effect the amount of leaf age 40 HST and 60 HST as well as the number of pods, planting seed weight , weight of 100 seeds, and fresh weight of plant root nodules. Application Rhizobium significantly affected the wet weight of stover. Trichoderma isolate Jati Jejer-02 capable symbio- sis with the plant so that the very significant effect on plant height age 7 HST and the amount of leaf age 20 HST. As well as the interaction between application Rhizobium and Trichoderma isolates significantly affected the number of pods plant.