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All Journal Jurnal Kedokteran Gigi Terpadu
Johni Halim
Departemen Biologi Oral, Fakultas Kedokteran Gigi Universitas Trisakti
Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Immunology & microbiology Medicine & Pharmacology

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Efek sitotoksisitas ekstrak etanol 70% curcuma xanthorrhiza roxb. Terhadap sel raw 264.7 yang diinduksi lipopolisakarida "lps" (Laporan Penelitian) Fika Alifiana; Monica Dewi Ranggaini; Johni Halim
Jurnal Kedokteran Gigi Terpadu Vol. 4 No. 2 (2022): Jurnal Kedokteran Gigi Terpadu
Publisher : Fakultas Kedokteran Gigi Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25105/jkgt.v4i2.15490

Abstract

Background: Curcuma xanthorrhiza Roxb. has bioactive compounds that are beneficial as anti-inflammatory. To get these compounds need to be extracted. Ethanol 70% can increase the solubility of both polar and non-polar compounds. RAW 264.7 cells with LPS induction cause an inflammatory state due to the active pro-inflammatory cytokines. To suppress the development of inflammation, a cytotoxicity test can be carried out to see the toxic nature of the extract against inflamed cells. Goals: To determine the cytotoxicity effect of 70% ethanol extract of C. xanthorrhiza on LPS-induced RAW 264.7 cells. Methods: In vitro laboratory experimental research. Preparation of extracts of C. xanthorrhiza Roxb. carried out with 70% ethanol then made concentrations of 200, 100, 50, 25, 12.5, 6.25, and 3.125 g/mL. Cytotoxicity was tested using the MTS. Untreated culture medium was used as a negative control and a positive control of diclofenac sodium. Then it was incubated for 24 hours at 37oC. After 24 hours of incubation, MTS was added to the plate and incubated again for 3 hours. The results were read using spectrophotometry. The resulting absorption is equivalent to living cells. The research data were analyzed using one-way ANOVA and Dunnet T3 tests. Results: Extracts of C. xanthorrhiza with concentrations of 200 and 100 g/mL were weakly cytotoxic and at concentrations of 50, 25, 12.5, 6.25, and 3.125 g/mL showed non-toxic effects on LPS-induced RAW 264.7 cells. Based on the IC50, C. xanthorrhiza Roxb. extract had a strong cytotoxic effect on LPS-induced RAW 264.7 cells. Conclusion: C. xanthorrhiza extract was toxic to LPS-induced RAW 264 cells.
Pengaruh ekstrak biji melinjo terhadap viabilitas dan apoptosis sel hsc-3 (Laporan Penelitian) Monica Dewi Ranggaini; Johni Halim; Richard Tridarmawan
Jurnal Kedokteran Gigi Terpadu Vol. 4 No. 2 (2022): Jurnal Kedokteran Gigi Terpadu
Publisher : Fakultas Kedokteran Gigi Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25105/jkgt.v4i2.15525

Abstract

Background: Squamous cell carcinoma (SCC) is the most common form of oral cancer. SCC treatment generally uses surgical procedures, chemotherapy, etc. Currently there are more than 60% of anticancer compounds obtained from natural ingredients, one of which is Gnetum gnemon L. (G. gnemon L.) or commonly called melinjo. Objective: To determine whether the G. gnemon L. seed ethanolic extract with concentrations of 1, 10, and 100 µg/mL could reduce viability and induce apoptosis in the HSC-3 cell line. Methods: Laboratory experimental research (in vitro) was conducted using the cell line HSC-3. Cells were treated with G. gnemon L. seed ethanolic extract with concentrations of 1, 10, and 100 µg/mL for 24 hours. Ethanol 70% was used as solvent and negative control. Doxorubicin was used as a positive control. The treated cells were analyzed using MTT assay and flow cytometry to examine changes in cell viability and apoptosis that occurred. Results: G. gnemon L. seed ethanolic extract with concentrations of 1, 10, and 100 µg/mL could significantly reduce viability and induce apoptosis in the HSC-3 cell line (p<0.05). The ability to reduce viability increased with the increase in extract concentration as many as 8,169 cells, 5,789 cells, and 3,068 cells and also induces apoptosis by 8.33 ± 0.93%, 16.55 ± 1.51%, and 28.73 ± 0.89% of cells tested sequentially. Conclusion: G. gnemon L. seed ethanolic extract with concentrations of 1, 10, and 100 µg/mL for 24 hours was able to reduce viability and induce apoptosis in the HSC-3 cell line.
Aktivitas Antioksidan Ekstrak Etanol Bunga Clitoria ternatea L. Dengan Senyawa Antioksidan (Antosianin dan Mirisetin) Monica Dewi Ranggaini; Johni Halim; Intan Paramitha Kumaladevi
Jurnal Kedokteran Gigi Terpadu Vol. 5 No. 1 (2023): Jurnal Kedokteran Gigi Terpadu
Publisher : Fakultas Kedokteran Gigi Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25105/jkgt.v5i1.16762

Abstract

Background: The COVID-19 pandemic is impacting a large number of populations. Patients with cancer infected with this virus require a longer treatment. Cancer occurs due to oxidative stress which creates an imbalance between the production of reactive oxygen species (ROS) and the amount of antioxidants in the body. Antioxidants are needed to balance ROS and increase the body's resistance to infection and disease. Clitoria ternatea L. is known to be one of the natural ingredients that has the potential as a natural antioxidant. It contains active compounds, such as anthocyanin and myricetin which act as antioxidants. Purpose: To find out the comparison of antioxidant activity C. ternatea L. extract in ethanol 70% solvent with the antioxidant compounds of anthocyanin and myricetin. Method: Research with laboratory experimental methods. C. ternatea L. simplicia was extracted using maceration method in 70% ethanol solvent. Antioxidant activity test using the 2,2-diphenyl-l-picrylhydrazyl (DPPH) method was carried out on samples of C. ternatea L. ethanol extract, anthocyanin and myricetin antioxidant compounds with a wavelength of 517 nm to measure their absorbance using a microplate reader. Result: The IC50 value of the ethanol extract of C. ternatea L. was 52.40 mg/mL, the antioxidant compound anthocyanin was 27.68 mg/mL and the antioxidant compound myricetin was 4.89 mg/mL. Conclusion: The antioxidant compound myricetin has very strong antioxidant activity compared to the ethanol extract of C. ternatea L. and the antioxidant compounds of anthocyanins.
Co-Authors Dewi Ranggaini Fika Alifiana Intan Paramitha Kumaladevi Richard Tridarmawan