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Wenny Tilaar
Program Studi Agronomi, Fakultas Pertanian, Universitas Sam Ratulangi, Manado
Agriculture, Biological Sciences & Forestry Economics, Econometrics & Finance Social Sciences

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Sterilisasi Dan Penggunaan Zat Pengatur Tumbuh Bap (Benzile Amino Purin) Terhadap Pertumbuhan Eksplan Tunas Pisang Abaka (Musa Textilis Nee) Melalui Teknik In Vitro Nathalia Unsong; Wenny Tilaar; Bertje R. A. Sumayku
AGRI-SOSIOEKONOMI Vol. 18 No. 3 (2022)
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (163.26 KB) | DOI: 10.35791/agrsosek.v18i3.44707

Abstract

This study aims to determine the most appropriate method and sterilization technique for the growth of abaca weevil shoot explants taken directly from the field and to determine the effect of the dose of growth regulator BAP (Benzile amino purine) on the growth of abaca feed. explants (Musa textilis Nee). through in vitro techniques. This study uses the multilevel sterilization method and the sterilization method using direct observation, namely when the explants have become shoots. The results showed that the appropriate sterilization method was a fixed sterilization method with bayclin treatment with a concentration of 30% for (30 minutes), 20% for (20 minutes), 10% for (10 minutes) with several techniques carried out so that the explant was successful. grow, namely in the 6th technique. Induction of shoots of abaka banana weevil explants in BAP treatment was best at 7 ppm treatment by producing 5 shoots. With the height of each shoot, namely: a (0.9 cm), b (0.3 cm), c (0.4 cm), d (0.4 cm), and e (0.5 cm).
Pengaruh Kinetin Pada Pertumbuhan Kecambah Brokoli Hibrida (Brassica oleracea Var. Green Magic) Dan Kandungan Sulforafan Pada Kultur In Vitro Monica Pamela Doodoh; Wenny Tilaar; Sesilia Wanget
AGRI-SOSIOEKONOMI Vol. 18 No. 3 (2022)
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (153.084 KB) | DOI: 10.35791/agrsosek.v18i3.45023

Abstract

This study aims to observe the growth in the sprouting phase and to observe the sulforaphane content in the growth stages of broccoli sprouts treated with kinetin on MS media. The research was conducted at the Laboratory of Biotechnology, Faculty of Agriculture, Sam Ratulangi University and the Laboratory of Department of Chemical Engineering, State of Malang. The study used a Completely Randomized Design (CRD) with 4 treatments and 9 replications, nam ely without kinetin, 0.5 ppm kinetin, 1 ppm kinetin, 1.5 ppm kinetin. The variables observed were the time of sprout formation, sprout height, sprout weight, sulforaphane content. The results of this study showed that the sulforaphane content was not affected by the height and weight of the sprouts but the kinetin concentration, the higher the concentration given, the higher the sulforaphane content.
Pengaruh BAP (Benzyl Amino Purine) Pada Pertumbuhan Brokoli Hibrida (Brassica oleracea Var. Valencia) Dan Kandungan Sulforafan Pada Kultur In vitro Dewi Chintya Tompo; Wenny Tilaar; Edy Fredy Lengkong
AGRI-SOSIOEKONOMI Vol. 18 No. 3 (2022)
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (163.874 KB) | DOI: 10.35791/agrsosek.v18i3.45025

Abstract

This study aimed to determine the effect of BAP on the growth of hybrid broccoli and the synthesis of sulforaphane content grown on MS media. Observations were made for 2 weeks, namely on the first day after planting until the 14th day after planting. This study used a completely randomized design with 4 treatments and was repeated 9 times. The results showed that on the first day after planting the hybrid broccoli had germinated. It was found that the highest Broccoli sprout height in the treatment without BAP (Control) was 8.82cm, the highest weight of Broccoli sprouts in the treatment without BAP (Control) was 0.17g, and the highest sulforaphane content in the 1 ppm BAP treatment was 6.56µg/g.
Co-Authors Bertje R. A. Sumayku Dewi Chintya Tompo Edy Fredy Lengkong Monica Pamela Doodoh Nathalia Unsong Wanget, Sesilia A.