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All Journal TEKNIK Al-Kimia Makara Journal of Science JOURNAL OF CHEMICAL NATURAL RESOURCES (JCNaR)
Juwono, Ariadne Lakshmidevi
Departemen Fisika, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Indonesia
Biochemistry, Genetics & Molecular Biology Chemistry Engineering Materials Science & Nanotechnology

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Journal : Makara Journal of Science

 1 
Analysis of Pelvic Bone of Ovariectomized Rats using Infrared and Raman Spectroscopies Fitriyawardhani, Nurwentari Dyah; Juwono, Ariadne Lakshmidevi; Mulyaningsih, Neng Nenden
Makara Journal of Science Vol. 26, No. 1
Publisher : UI Scholars Hub

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Osteoporosis is a bone disease that generally occurs in postmenopausal women. Osteoporosis can be studied using an animal model such as rats because rat bone structure is similar to human bone structure. This research aimed to analyze pelvic bones of ovariectomized rats that showed osteoporosis signs for the first time. In this study, 20 Rattus norvegicus were given ovariectomy at 12 weeks of age and were used as an animal model for menopausal women. The rats were euthanized every 2 weeks from 13 to 21 weeks of age. In addition, the pelvic bones of ovariectomized rats will be analyzed using FTIR and Raman spectroscopy to show the first osteoporosis signs periodically. Results of FTIR and Raman spectroscopy indicated that the pelvic bones of the ovariectomized rats showed the first osteoporosis signs at 17 weeks of age with changes in phosphate and carbonate contents, increased hydroxyl functional groups, reduction of mineral maturity about 7.47%, and reduction of crystallinity about 29.26%. In addition, morphological changes from fine fibers to coarser fibers and reduction of the crystal size and crystallinity index at the angle of 2θ ≅ 25° about 9.29% and 25.18%, respectively, were observed using SEM-EDS and XRD.
Antibacterial Testing on Silver/Zinc Oxide Nanoparticles/Organoclay Reinforced Chitosan Biocomposites Muiz, Lisna Junaeni; Juwono, Ariadne Lakshmidevi; Paputungan, Zulkarnaen; Krisnandi, Yuni Krisyuningsih
Makara Journal of Science Vol. 27, No. 3
Publisher : UI Scholars Hub

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Herein, bionanocomposites of chitosan (CS)/silver nanoparticle/organoclay/zinc oxide nanoparticle (CS/Ag/OC/ZnO) were prepared for antibacterial food packaging. This study examines the time variation in the AgNP synthesis method by comparing local (74˗85% deacetylated) and commercial chitosan (75%˗85% deacetylated) as a reducing and capping agent and seeks to reconstruct the optimum ratio formulations of AgNPs and ZnONPs in bionanocomposites for food packaging. The results reveal that the synthesis of AgNPs was successfully carried out using a local chitosan solution as a reducing and capping agent. The CS/Ag/OC/ZnO films exhibit structural, mechanical, and optical properties suitable for food packaging and antibacterial activity on Staphylococcus aureus and Escherichia coli. The relative inhibition zone increased with increasing numbers of AgNPs and ZnONPs. The inhibition zone values indicated that E. coli and S. aureus bacteria were sensitive to the film, namely 12.5 ± 1.5 mm, and 16 ± 0.0 mm, respectively.
Co-Authors Anawati, Anawati Asraf, Ahmedi Chambioso, Yugo Daulay, Amru Fitriyawardhani, Nurwentari Dyah Franciska Pramuji Lestari Ika Kartika Jihad, Bagus Hayatul Muiz, Lisna Junaeni Mulyaningsih, Neng Nenden Nugraha Thaha, Yudi Paputungan, Zulkarnaen Yuni Krisyuningsih Krisnandi, Yuni Krisyuningsih