Yuda C Hariadi, Yuda C
Physics Department Faculty of Mathematics and Natural Sciences, University of Jember, Jember68121, East Java, Indonesia

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Lead Distribution on Marsilea crenata and Its Effect on Growth and Total Protein Content Nurhayati, Arry Y.; Hariadi, Yuda C
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UNEJ e-Proceeding

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The increasing risk of lead poisoning such as neuro-behavioral disorders and neurological damage; hypertension, brain and kidney damage; DNA and antioxidant defense system cell damage in developing countries due to the increase in lead contaminated environment. This research was aimed to investigate the effects of prolonged lead on protein levels in the Marsilea crenata plant. The plants have been used for generation by local farmers as a daily source of vegetables during crop growing season. The plants are also known as an exotic cuisine known as Semanggi Surabaya. Marsilea crenata were grown inside a glasshouse using a modified Hoagland solution in different levels of Pb. The growth, Pb content, and total protein content were then measured for eight weeks during exposure. The result has shown that the root of Marsilea crenata has the capability to absorb lead and translocate them inside its shoots and leaves, with the biggest amount were translocated to the younger leaves during one week of exposure and was then accumulated in the old leaves after longer exposures. Total protein content was higher on younger leaves than in older leaves, followed by shoot and then the root. The result has shown that with deep knowledge of Marsilea crenata it is possible of using it as a phytoremediator or as a source of food since the total protein was translocated on the edible part. Still, awareness is still needed since the plant may contain high levels of Pb with the existence of stress symptoms. It should be avoided to reduce the risk of lead poisoning. Keywords: Lead, Marsilea crenata, growth, protein
Susceptibility of Two Varieties Rice (Oryza sativa L.) on Saline Environment Hariadi, Yuda C; Nurhayati, Arry Y; Endraningrum, A.
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UNEJ e-Proceeding

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Abstract

Serealia such as rice, wheat, and maize are very important world food demands, and rice was highest on the nutrional protein status with respect to amino acid composition. Rice (Oryza sativa) has been an important primary cereal for generations that supply around 90% of carbohydrate needs in Indonesia, that every effort to be made in acquiring the fullfillment demand. The research was aimed to investigate the tolerance of two rice varieties Towuti and Ciherang grown on fertile and arid soil on different salinity. Healthy and homogenous three weeks old seedlings were grown in a glasshouse on 0, 50mM, 100mM, 200mM, and 300mM of NaCl on plastic pots with two different soil, under a randomized design and five replicated five times. The growth in terms of leaf area and surface electrical potential difference were observed on a weekly basis for four weeks after the plants had been exposed. The result showed that leaf area and the electrical PD of both rice varieties were significantly reduced under fertile and arid soil under 200mM of NaCl and 300mmM of salinity. It is implied that the photosynthetic process was significantly affected by salinity and was concluded that both varieties were sensitive to salinity at 200mM of NaCl. There was possibility that the protein content was involved on the osmotic change on the salinity process, element supplements were needed, but there is a need for less expensive methods. Keywords: salinity, rice; protein, electrical PD, growth