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Rudi Harianto
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Agriculture, Biological Sciences & Forestry Environmental Science

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MODIFIKASI MEDIA ALTERNATIF DARI SAYURAN UNTUK ANALISIS KUANTITATIF PERTUMBUHAN MIKROORGANISME ASAL TANAH GAMBUT KALIMANTAN BARAT DENGAN METODE TPC Rezekikasari Rezekikasari; Rudi Harianto
Perkebunan dan Lahan Tropika Vol 9, No 1 (2019)
Publisher : Fakultas Pertanian Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (231.697 KB) | DOI: 10.26418/plt.v9i1.37718

Abstract

Medium formulations for quantitative testing and isolation of microorganism from peat soils, require adequate nutrition. This needs to be supported by the type of substrate and incubation conditions that support each other. Isolation media used mostly though available, but the price is quite high. This is what underlies the need to find alternative sources of new substrates that can support the growth of microbes from peat soils. Some of these ingredients include, carrots, Gembili Bulbs, tomatoes, and pumpkin which are abundantly available in West Kalimantan. This study shows that the best substrate in isolation of bacteria and fungi from peat is Gembili Bulbs with a total colony of 26 x 105 (CFU / gr) and fungi of 10 x 105 (CFU / gr). Peat bacteria and fungus cannot grow on carrot and pumpkin substrate media as indicated by the absence of growing microbial colonies. The best formulation of microbial isolation media with Gembili Bulbs is also supported by the highest C / N ratio of 44.8.Key Words : Bacteria, Fungi, Quantitative, Pour Plate Methode, TPC
Co-Authors Rezekikasari Rezekikasari