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MORPHOLOGICAL AND CHROMOSOMAL CHARACTERIZATION OF ORCHID Peristylus goodyeroides Lindl. FROM CURUG SETAWING, KULONPROGO Kurniawan, Febri Yuda; Riyadi, Anindita Della Rosa
Jurnal Pendidikan Matematika dan IPA Vol 12, No 2 (2021): July 2021
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (571.851 KB) | DOI: 10.26418/jpmipa.v12i2.41811

Abstract

Peristylus goodyeroides is a terestrial orchid that scattered around Southeast Asia. Morphological characters of P. goodyeroides can vary, depending on the ecological factors and habitat. Cytological characters in the form of chromosome configurations can be used as a taxonomic tool for the process of identifying and understanding variations in taxa. The purpose of this study was to determine the morphological characters and chromosome configuration of the P. goodyeroides from Curug Setawing, Kulonprogo. The method used was morphological characterization and plant chromosome preparation by squash method with the main steps of fixation, maceration, staining and observation. Data were analyzed with the help of Image raster 3, Corel Draw X7, and Microsoft Excel 2013. P. goodyeroides from Curug Setawing has the morphological characters of root tubers, cylindrical stems, ovate leaf shape, convolutive leaf arrangement and creamy white flowers. The orchid has a number of chromosomes 2n = 10 with a karyotype formula of 2n = 2x = 8m + 2t. Metacentric chromosomes are found on chromosomes 1-8 and telocentric chromosomes 9-10. The absolute arm length of the chromosomes has a range of 2.03-3.44 μm, the relative arm length of the chromosomes is 2.21-3.32 μm, the length of the p arm is 1.13-1.58 μm and the q arm is 1.23-2.12 μm.
Effect of Alkaline and Drought Stress on Growth and SOD (Superoxide dismutase) Content in Basil Plant (Ocimum americanum L.) Riyadi, Anindita Della Rosa; Siswanti, Dwi Umi
Jurnal Biodjati Vol 7 No 1 (2022): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v7i1.16680

Abstract

Basil plant (Ocimum americanum L.) is a fragrant annual plant widely cultivated by the people in Indonesia because it is useful and commercial. Previous research on salinity and drought stress in basil affects growth and development plant, cell turgor pressure, and the anatomical structure of the plant. However, research on the effect of alkalinity and drought stress in basil plants has not been carried out. Therefore, this study was conducted to determine the effect of variations in alkaline and drought stress on the growth and superoxide dismutase (SOD) content of basil plants. Alkalinity stress was carried out by variations of dolomite (D) doses which is divided into four levels, namely D1, D2, D3, and D4 (0 grams/pot, 100 grams/pot, 150 grams/pot, and 200 grams/pot) and variations of drought stress were divided into four types of field capacity, namely A1, A2, A3, and A4 (25%, 50%, 75%, and 100%). Parameters measured were plant height, leaf length and width, number of leaves, fresh and dry weight, SOD content, and environmental parameters. The growth of the basil plant (O. americanum L.) decreased after being given drought stress at a field capacity level of 25%, while the SOD content of basil (O. americanum L.) did not differ significantly but increased with the increase in dolomite doses and field capacity given.