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All Journal Jurnal Ilmu Kelautan Spermonde Biosaintifika: Journal of Biology & Biology Education
Nia Kurniawaty, Nia
Faculty of Agriculture, Bogor Agricultural University, Indonesia
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences

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Characterization of Three Species of Thrips on Weeping Fig, Nutmeg, and Marine Seruni Plants Based on Mtcoi DNA Sequences Kurniawaty, Nia; Hidayat, Purnama; Rauf, Aunu
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 2 (2016): September 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i2.5448

Abstract

Thrips are widely reported as pests in vegetable crops. However, the existence of Phlaeothripidae members has a less concern in Indonesia. Phlaeothripidae is the only family of Tubulifera Suborder and some reports suggested that they had potential to be pests in several crops due to their ability to roll up and to make galls on leaves. The first step in pest management attempt is to identify the pest accurately and quickly, so the pest management can be on target and more efficient. One of the identification methods is the molecular identification using DNA barcoding techniques. This study aimed to characterize and to compare species thrips in banyan, nutmeg, and marine seruni based on their molecular characteristics. This research was conducted in Bogor and Kuningan. The process of molecular characterization consisteds of four steps DNA total extraction, amplification by using PCR, COI gene sequence, and data analysis. PCR programme was succesfully to amplified mtCOI gene fragment at 710 bp. The length of mtCOI gene of Gynaikothrips uzeli, Haplothrips ganglbaueri, and Pseudophilothrips ichini were 704, 686, and 702 bp dominated by A and T bases with nucleotide variation value of 27.8%. This results confirmed that molecular characterization using mtCOI gene mitochondrial had successfully supported the morphological data.How to CiteKurniawaty, N., Hidayat, P. & Rauf, A. (2016). Characterization of Three Species of Thrips on Banyan, Nutmeg, and Marine Seruni Plants Based on Coi Gene. Biosaintifika: Journal of Biology & Biology Education, 8(2), 185-192.
HUBUNGAN KONDISI TERUMBU KARANG DENGAN KELIMPAHAN IKAN KARANG TARGET DI PERAIRAN PULAU TINABO BESAR, TAMAN NASIONAL TAKA BONERATE, SULAWESI SELATAN Ghiffar, Muhammad Albar; Irham, Andi; Harahap, Syawaludin A.; Kurniawaty, Nia; Astuty, Sri
Jurnal Ilmu Kelautan SPERMONDE VOLUME 3 NUMBER 2, 2017
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/jiks.v3i2.3002

Abstract

This research aims to understand the condition of coral reef (life coral), the abundance of reef target fishes, and the corellation between those two variables. This research has been conducted in Tinabo Besar Island with 6 sampling stations in 5-6 meter depth. Data collected are substrate cover precentage (life form), and the number of reef target fish appeared from 7 specified families (Serranidae, Lutjanidae, Haemulidae, Lethrinidae, Scaridae, Siganidae, and Acanthuridae) using Underwater Photo Transect (UPT) and Underwater Visual Census (UVC). Substrate cover analyzed using CPCe software with life coral, dead coral, algae, abiotic, and other biota cover output. Mortality Index (MI) obtained from the ratio of life coral and dead coral. The result shows that percentage of life coral coverlie between 11.87% to 38.80%, with dominance of Coral Masive (CM). It’s mean that the coral reef condition is in poor to moderate category. Coral death ratio is low with MI between 0.15 to 0.30. Reef target fish total abundance is 493 individual/2100m2 from 31 species, with dominance from Lutjanidae family (173 individual). The result shows positive and strong enough correlation between coral reef coverage and reef target fish abundance (r=0.65) with Determinantion Coefficient at 42.55%. Linear regression is y=-12.929+3.7562x, where in every addition of coral reef cover percentage, resulted in addition of 4 fishes.Keyword: life coral, abundance, reef target fish, correlation, Tinabo Besar Island
Co-Authors Ghiffar, Muhammad Albar Irham, Andi PURNAMA HIDAYAT RAUF, AUNU Sri Astuty Syawaludin A. Harahap