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M Irwan Sapta Purna Bintang
Marine Science Study Program, Postgraduate, Universitas Riau
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences Environmental Science Immunology & microbiology Social Sciences

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Identification of Saponins from the Active Fraction Sea Cucumbers (Stichopus vastus sluiter) M Irwan Sapta Purna Bintang; Mery Sukmiwati; Rahman Karnila
Jurnal Perikanan dan Kelautan Vol 28, No 1 (2023): February
Publisher : Faculty of Fisheries and Marine, Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jpk.28.1.112-115

Abstract

This study aims to determine the secondary metabolic content of saponin contained in the active fraction of sea cucumber (Stichopus vastus) by using metabolic test reagents and thin-layer chromatography (TLC) analysis. The active fraction was given the reagent in the form of HCl 2N and strong acid then continued with analysis to determine the Rf value and lieberman-burchad reaction. The results of the metabolic reaction test showed that the active fraction contained steroidal saponin compounds characterized by the presence of a stable foam and the formation of a greenish-green color. TLC analysis produced a single stain with an Rf value of 0.9 and a bluish color reaction after being given Liberman-burchad which confirmed that the saponins contained in the active fraction of sea cucumbers were steroidal saponin
Co-Authors Karnila, Rahman Karnila Mery Sukmiwati