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Mery Sukmiwati
Marine Science Study Program, Postgraduate, Universitas Riau
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences Environmental Science Immunology & microbiology Social Sciences

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Identification of Saponins from the Active Fraction Sea Cucumbers (Stichopus vastus sluiter) M Irwan Sapta Purna Bintang; Mery Sukmiwati; Rahman Karnila
Jurnal Perikanan dan Kelautan Vol 28, No 1 (2023): February
Publisher : Faculty of Fisheries and Marine, Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jpk.28.1.112-115

Abstract

This study aims to determine the secondary metabolic content of saponin contained in the active fraction of sea cucumber (Stichopus vastus) by using metabolic test reagents and thin-layer chromatography (TLC) analysis. The active fraction was given the reagent in the form of HCl 2N and strong acid then continued with analysis to determine the Rf value and lieberman-burchad reaction. The results of the metabolic reaction test showed that the active fraction contained steroidal saponin compounds characterized by the presence of a stable foam and the formation of a greenish-green color. TLC analysis produced a single stain with an Rf value of 0.9 and a bluish color reaction after being given Liberman-burchad which confirmed that the saponins contained in the active fraction of sea cucumbers were steroidal saponin
Functional Groups of Scale Collagen from Farmed Snakehead Fish (Channa striata) and Wild Sources Ayu Agustina; Bustari Hasan; Mery Sukmiwati
Jurnal Perikanan dan Kelautan Vol 28, No 2 (2023): June 2023
Publisher : Faculty of Fisheries and Marine, Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jpk.28.2.279-283

Abstract

The study aimed to analyze the functional groups of scale collagen from farmed snakehead fish and wild sources. Scale collagen from farmed snakehead fish was higher than the wild sources (18.82 > 14.27%) with the protein content of both successively (60.24 > 59.78%). The functional groups in scale collagen from farmed snakehead fish were Amide A (3668,77 cm-1), Amide B (2920,35 cm-1) Amide I (2302,14 cm-1), Amide II (1446,67 cm-1), and Amide III (1245,10 cm-1). Meanwhile, scale collagen from wild sources snakehead fish has an Amide A group (3853.94 cm-1), Amide B (2905.89 cm-1), and Amide I (1869.10 cm-1) Amide II (1457.28 cm-1), Amide III (1243.18 cm-1). Scale Collagen from farmed snakehead fish and wild sources is classified as type 1 collagen
Co-Authors Ayu Agustina Bustari Hasan Karnila, Rahman Karnila M Irwan Sapta Purna Bintang