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Lamees A. Abdul-Lateef
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Medicine & Pharmacology

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Molecular Detection of some Virulence Genes of Streptococcus agalactiae Isolated from Pregnant Women Lamees A. Abdul-Lateef
Journal of Global Pharma Technology Volume 11 Issue 2: 2019
Publisher : Journal of Global Pharma Technology

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Abstract

virulence factors like surface proteins, biofilm, and exotoxins s. All these allow bacteria to adhere to tissues causing pathogenesis and invade the immune system causing serious infection. Materials and Methods: A total of 300 samples, 25 of Strep. Agalactiae were collected in this study. Strep. Agalactiae were isolated from pregnant women by standard bacteriological methods. PCR was conducted to determine the some virulence genes of the isolates by using primers cyle, scpB, and hylB. The PCR amplification products were visualized by electrophoresis on 1% agarose gels for 30min at 70v. The size of the amplicons was determined by comparison to the 100 bp allelic ladder. Results: In this study, 25 isolates of Strep. agalactiae were genotypically characterized by PCR assay. Among isolates studies it was found that cylE gene present in all isolates. Also 100% of isolates have ability to produce scpB gene while only 11(44%) was able to produce hylB.Keywords: Streptococcus agalactiae, cylE, scpB, hylB.
Sequencing of Proteus Toxic Agglutinin (Pta) Gene in Proteus Mirablis and Cytotoxic Effect of Pta on Human Colon Cancer Cell and Human Kidney Cell Lamees A. Abdul-Lateef
Journal of Global Pharma Technology .
Publisher : Journal of Global Pharma Technology

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Objective study the sequencing of Pta gene and the cytotoxic effect of pta gene on SW480 and ACHN cell line Material and method detection of pta gene by using PCR technique, detection of mutation in the Pta gene by used automated sequencing. Also the cytotoxic effect of Pta toxin on cell lines of SW480 and ACHN cell were used in vitro. Results the result was revealed that the Pta gene is present in 17 isolates (15 isolated from urine and 2 isolates from stool).  Sequencing of Pta gene from isolates of Proteus mirablis reveals of 43 mutations in five isolates and gives identity in percentage (92-95%) with standard strand in NCBI web site. However, the cytotoxic effect of Pta on cells line of SW480 and ACHN were used in vitro was also investigated. The result was shown that the effect of Pta toxin on SW480 lead to the proliferation of cell was significantly elevated when Pta toxin at 12.5%, 25%, and 50% concentrations. The concentration 50% of Pta toxin a significantly better effect on cell growth On the other hand, ACHN  cell  was gradually increase in growth at concentrations (6.25%, 12.5%%, 25%, and 50%)Keywords: Proteus toxic agglutinin gene, Sequencing, Human colon cancer cell line, Human kidney cell line.
Molecular Detection of Some Virulence Genes of Salmonella Typhymurium and Salmonella Enteritidis Isolated from Patients Suffering Watery Diarrhea Lamees A. Abdul-Lateef
Journal of Global Pharma Technology Volume 11 Issue 05.
Publisher : Journal of Global Pharma Technology

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Abstract

Salmonella enterica virulence factors can be divided into chromosomal, plasmid and bacteriophages encoded virulence factors. Many of the Salmonella virulence factors, such as adhesion, invasion, and toxin genes are clustered in certain areas of the chromosome known as “Salmonella pathogenicity islands” (SPI). A total of 200 clinical stool samples were collected during this study which obtained from patient Suffering from watery diarrhea who a dmintted to three main hospitals of Babylon Governorate: Merjan Medical city, Al-Hillah Surgical Teaching Hospital and Babil Teaching Hospital for Women and Children during a period of three months (from july 2018 to September 2018). Out of 200 specimens 35(17.5%) were detected by culture and vitek2 compact, 24 (12%) of them were confirmed by PCR using target gene, (17) Salmonella typhimurium and (7) Salmonella enteritidis. PCR was conducted to determine the some virulence genes of the isolates by using primers inv A, sdiA and sifA. The PCR amplification products were visualized by electrophoresis on 1% agarose gels for 35min at 70v. The sizes of the amplicons were determined by comparison to the 200 bp allelic ladder. Among isolates studies it was found that invA gene present in 13(54)% isolates of S. enterica ,(9) isolates of S. typhimurium and (4) isolates of S. enteritidis , sdiA gene it was found in all isolates (100)% while sifA gene it was found in all isolates only isolate number 18 not contained this gene 23(96)%.Keywords: Salmonella typhimurium, S. enteritidis, invA, sdiA and sifA.
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