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Frial Gemeel Abd
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Medicine & Pharmacology

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Isolation and Identification of Bacterial Causes of Acute Tonsillitis Frial Gemeel Abd
Journal of Global Pharma Technology .
Publisher : Journal of Global Pharma Technology

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Abstract

Acute tonsillitis was an inflammatory process of the tonsillar tissues and was usually infectious in nature. Acute tonsillitis starts suddenly and usually goes away again within one to two weeks. This infection was often bacterial, or sometimes viral.   A hundred of blood and swabs specimens were collected from patients who were suffering from tonsillitis whose did not take any drugs for both sex with age ranging from 1-60 years in AL-Hilla-Teaching hospital and hospital Babylon for birth and children during a period from October 2017 through January 2018 . The causative agent for tonsillitis was   isolated and diagnostic of microorganism according to morphological and biochemical test  by using VITEK compact 2 .The results  appeared the number of gram positive bacteria about 100 and 23 isolate from  gram negative bacteria .The most common causative agent was Streptococcus pyogenes and Staphylococcus aureus. The sensitivity of bacterial to antibiotic was appeared most of bacterial resistance to amoxicillin, Cefotaxime and Aztreonam) but sensitive to other antibiotic such as Gentamycin, Chloramphenicol and Ciprofloxacin kit. Qualitative and semi quantitative ASO determination was done using the ASO latex agglutination kit. 24%of patient was ASO positive and 67% was ASO negative The level of ASO in patients that tested positive for the presence of ASO ranged from 1600IU/ml to 25600IU/ml. 12 sample were the highest concentration of 25600IU/ml .The conclusion of this study found that Streptococcus pyogenes was most common causative agent for tonsillitis and the bacteria resist amoxicillin.Keywords: Tonsillitis, Amoxicillin, Streptococcus, ASO.
Exotoxins A, T and S among Pseudomonas Aeruginosa Isolates from Clinical and Hospital Environment Samples Ahmed Jamal Hussein; Frial Gemeel Abd
Journal of Global Pharma Technology Vol. 9 Issue 7 : 2017
Publisher : Journal of Global Pharma Technology

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Abstract

Pseudomonas aeruginosa is one of the most widely recognized Gram-negative   opportunistic pathogens related with nosocomial infections. The presence of several virulence factors, for example, exotoxins of the bacteria making a genuine risk to the patient. This study was intended to detect Exotoxin A, T and S genes in P. aeruginsoa isolates utilizing PCR system. It includes (30) isolates of bacteria diagnosed by traditional techniques and confirmed by the API 20Eand Vitek 2 Systems  , DNA isolated and subjected to amplify using specific primers for bacteria. The consequences of PCR amplification for the genes encoding for exotoxin A, T and S demonstrated that all P. aeruginosa isolates showed the presence of the genes encoding for exotoxin A and exotoxin T corresponding to 100 % of the isolates while twenty two (22) isolates comparing to 73.33 % of the total thirty (30) isolates were recorded to be positive for exotoxin S gene . It can be concluded that exotoxins are a conspicuous virulence factors for P. aeruginosa and responsible for a genuine threat to patients.Keywords: Exotoxin, Pseudomonas aeruginosa ,Toxin ,Environmental Isolate. 
Co-Authors Ahmed Jamal Hussein