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Ahlam Kadhum Al-Yasseen
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Medicine & Pharmacology

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Phenotypic and Genotypic Characterization of Some Virulence Factors among Proteus mirabilis Isolated from Clinical Samples in Al-Najaf Al-Ashraf/ Iraq Ahlam Kadhum Al-Yasseen
Journal of Global Pharma Technology Volume 11 Issue 05.
Publisher : Journal of Global Pharma Technology

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Abstract

The present study aimed to investigate the phenotypic and genotypic pattern of some virulence factors in Proteus mirabilis isolates from clinical samples. A total of 110 urine samples were collected from patients with urinary tract infection from both sexes and 100 vaginal swabs were collected of women with vaginal infections during the period from October 2017 to January 2018. The results of primary isolation and identification of bacterial isolates depending on microscopic, culturing on CHROM agar and biochemical test showed that 36.3% of isolates were belong to Proteus while the results of PCR techniques that used to confirmed identification using 16SrRNA revealed that 27.2% of isolates were belong to Proteus mirabilis. Variable results in the ability of bacterial isolates to produce protease and hemolysin have been obtained in which 30% and 100% of bacterial isolates gave positive results respectively. Also the results of PCR technique showed that all isolates (100%) were possess zap A, rsb A and mrp A encoding for the production of protease, protein that regulates  bacterial swarming behavior and mannose resistant pilli (MR /P) respectively by the appearance of amplicons with molecular weight  552, 485 and 366 bp.Keywords:  Genotypic; Proteus mirabilis; Phenotypic; and Clinical Samples.
Distribution of Fim A Genotypes among Porphyromonas Gingivalis associated with Chronic Periodontitis in Al-Najaf Al-Ashraf City Ahlam Kadhum Al-Yasseen
Journal of Global Pharma Technology Volume 10 Issue 05: (2018) May2018
Publisher : Journal of Global Pharma Technology

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Abstract

This research aims to detect the prevalence of fim A genotypes I, Ib, II, III, IV and V among Porphyromonas  gingivalis related with periodontitis. Amplification of 16S rRNA gene showed that out of 150 DNA samples that extracted from subgingival plaque specimens of patients with chronic periodontitis only 40 DNA specimens were belonged to P. gingivalis and DNA sequencing demonstrated 98% homologous with P. gingivalis strain CP38 16S rRNA gene, partial sequence. fimA type IV gene was the most predominance with 27.5% followed by fimA type III with12.5%  while fimA type II show up with the lowest percentage (5%) while fimA type Ib was not identified. This finding denoted that  P. gingivalis that possess  fimA type IV was the most importance pathogens associated with  chronic periodontitisKeywords: P. gingivalis, Fim A genotypes, Periodontitis.
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