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R. A. Sahib
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Medicine & Pharmacology

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Isolation and Dignosis of Penicillium Expansum from the Cosmetics Which Ability to Produce Patulin and using Zinc Oxide Nanoparticles as Antifungal Compound R. A. Sahib
Journal of Global Pharma Technology Volume 11 Issue 2: 2019
Publisher : Journal of Global Pharma Technology

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Abstract

 In this study Penicillium expansum was isolated from the cosmetic samples, 50 cosmetic samples which were used such as (eye shadow, face cream, face mask, lipstick and  hand cream). The sabouraud dextrose agar was used to test  the presence of  P. expansum.  Thin layer chromatography (TLC) was appeared yellow spots of patulin toxin with rate of flow (RF) is 76% under UV light exposure.The UV-visible spectroscopy result proved the wavelength of patulin is located with 270 nm and 1.8 absorbance.High performance liquid chromatography (HPLC) results show that retention time of major peak of standard patulin and a filter extract of P. expansum are  located at (15 and  15.1) min, respectively,  which are located  in the same area. Zinc oxide  nanoparticles using as a fungicide to control development growth of P. expansum which proved high efficiency to decrease growth of P. expansum by percentage of (33,50,77) % respectively in the concentration (2,4,6) mmol/L, while 8 mmol/L of ZnO caused complete inhibition growth of P. expansum as compared with the control group which untreated with ZnO NPs. Scanning electron microscopy (SEM) images show that ZnO NPs caused decreased with disrupted of conidia and decreased number of  mycelium.Keywords: Penicillium expansum, Cosmetics, UV-vis, HPLC, SEM, Zinc oxide nanoparticles.
Isolation and Diagnosis of Fungi Which Producing Aflatoxin B1in Chips in Local and Imported Markets Najaf Province R. A. Sahib
Journal of Global Pharma Technology .
Publisher : Journal of Global Pharma Technology

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Abstract

This study included isolating and diagnosing contaminated fungi from some local and imported chipsin Najaf Province,and to detect their ability to produce aflatoxin B1. The results showed that highest percentageof visible fungi namely are Aspergillusflavus, A. niger, A. glaucus, Alterneria sp., Fusarium sp. and A. terreusith ratio are (100, 95, 92,80, 75, 5.7) % respectively in Wejoh chips and these fungi appeared in all types of chips under study. The results of the frequency of the same fungi in the wejoh chips are (80,70, 68, 52, 50,2) % respectively.A.flavus is more visible and frequently in thesame types of chips,and preferable sabouraud dextrose (agar,broth) medium to growth. Primary result proved that  A. flavus producing aflatoxin B1in Thinlayerchromatography (TLC) which appear blue spot of aflatoxin B1 with rate of flow (RF) is 56 % under UV light expose with wavelength of (365 nm).High performance liquid chromatography (HPLC) results show that retention time or major peak of standard aflatoxin B1 located at 10.54 minutes, while a filter extract of A. flavusis10.75 which  identifies with aflatoxin B1 standardwhich located  in the same area.Keywords: Chips, A. flavus, Aflatoxin B1, SEM, TLC, HPLC.
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