Sara K. Al-Zubaidi
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Molecular Identification of Extended-spectrum β-lactamase Resistant Genes in E coli Recovered from Individuals Suffered Urinary Tract-based Infection Sara K. Al-Zubaidi
Journal of Global Pharma Technology Volume 11 Issue 2: 2019
Publisher : Journal of Global Pharma Technology

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The intended purpose of the current identifying study was to know the recent occurrence of E. coli-related extended-spectrum resistant-genes of blaCTX-M, blaSHV, blaTEM and blaAMPC that are responsible for community-based acquired urinary tract infection (CAUTI) and nosocomial infections (NCI). The study also was intended to reveal the genetic-based links between recovered strains. To answer these questions, non-similar 50 isolates of E. coli were recovered from UTI samples handed by 3 hospitals in Babylon City, Iraq. These samples were subjected to cultivation processes and a polymerase chain reaction technique (PCR). The resistant genes, according to PCR, were detected in 10 positive-ESBL phenotypes of the isolates. Using PCR, The blaCTX-M and blaAMPC genes were 100%-frequently identified in those 10-positive samples. However, the other genes were detected in low rates in those samples. The results, here, identify the important role of the horizontal gene transfer (HGT) in obtaining resistance by the E. coli isolates against β-lactamase-related antibiotics in Iraq.      Keywords: E. coli, Extended, β-lactamase resistant genes, Spectrum.
Evaluation and Investigation of the Prevalence of Superficial Mycosis among Primary Schools Pupils in Al-Dewaniyah Governorate, Iraq Sara K. Al-Zubaidi
Journal of Global Pharma Technology Volume 11 Issue 04.
Publisher : Journal of Global Pharma Technology

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Background: The Superficial mycosis infections are the widespread types of mycosis among children primary schools. Objective: To detection and evaluation the extent and prevalence of superficial mycosis infection among primary school pupils in Al-Dewaniyah province. Subjects and methods: The survey included the selection of the pupils were randomly selected based on those with tinea (ringworm) lesions sample of 103 pupils (males 81, females 22) respectively from ten primary schools (six male and four female schools), they clinically diagnosis dermatophytoses, in the Diwaniyah Teaching Hospital, Dermatology unit, during a period from the beginning of December 2018 till the final February 2019. The specimens were collected in sterile plastic Petri dishes or sterile plastic envelopes and consisted of skin scales, hairs, or clippings from fingernails and toenails. The samples of fingernails and toenails were cut or to get mycelial elements. Results: The results of identity and incidence of the dermatophytes and non-dermatophytes isolated were revealed that dermatophytic isolation " Tinea" was 36/103(34%) among patients and this percentage was distributed to various clinical cases of ringworm ''tinea'', Mean age of patients enrolled in the present study was 20.11+10.99 years with a range of 7 to 13 years, According to gender patients were classified into 25 male s (69%) and 11 females (31 %). dermatophytes infections Is a problem in the health system of many developing countries and influences all ages chiefly primary pupils.  Conclusion: The current study prevalence of superficial mycosis among pupils of primary school was significant risk factors assessed such as poor personal hygiene, overcrowding. Consequently, proper health education, personal and environmental sanitation, provision of infrastructure, primary health care and regular clinical studies are recommended for these primary school pupils.Keywords: Dermatophytes, Primary school pupils, Mycelia, Superficial mycosis.