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Ilham A. Bunyan
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Medicine & Pharmacology

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Molecular Identification and Phenotypic Detection of Some Virulence Factors among Aeromonas hydrophila Isolated from Diarrhea Cases (Iraq) Ilham A. Bunyan
Journal of Global Pharma Technology .
Publisher : Journal of Global Pharma Technology

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Abstract

The present study included the detection of Aeromonas hydrophila in clinical samples were taken from patients suffering from diarrhea during the period from July (2017) to October (2017). A. hydrophila isolates which were diagnosed by four different methods (Culture media, biochemical tests, Api20E system and Polymerase chain reaction (PCR). Api20E kit was the important method for diagnosis of bacteria, which has led to isolate and, diagnosis of (35) isolates of A. hydrophila, but the PCR method of gene (16S rRNA) was more accurate method for diagnosis, which has led to isolate and diagnosis as (16) clinical isolates. The virulence factors of bacteria were detected, and showed that all isolates have ability to produce, heamolysin, protease, lipase, and capsule formation but have no ability to produce siderophores. In addition, A. hydrophila have the ability to adhere to uroepithelial cells of human and have strong biofilm formation.Keywords: Aeromonas hydrophila, 16S rRNA gene Capsule, Sidrophore, hemolysin, and Lipase.
Molecular Detection of Gardnerella Vaginalis Isolated from Preterm Labor Patients and Study of Some Factors on Biofilm Formation in Al-Hilla City, Iraq Ilham A. Bunyan
Journal of Global Pharma Technology Volume 09 Issue 12
Publisher : Journal of Global Pharma Technology

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Objectives: the present study aims to isolation and identification of G.vaginalis from preterm labor, detection of bio film formation of G.vaginalis and effect of some factor on bacterial adherence. Material and methods: One hundred fifty clinical sample were collected from preterm labor patients with (bacterial vaginosis, urinary tract infection and aborted women) 80, 15, 55 respectively, admitted to Babylon Maternity and pediatric hospital and Al-Hilla Teaching Hospital, at the period from February to October 2016. These high vaginal samples were subjected to different methods of identification of G.vginalis. Results and conclusion: It was found that 6 (4%) G.vaginalis isolates were recovered by using selective media where 30 (20%) isolates were recovered dependent on direct extraction to high vaginal swab on molecular level.  We  were  detection and found  that  (4) isolates were gave strong biofilm While the other Gave moderate then subjected to some factors to reduce , The effect (Alum ,sodium bicarbonate  and sodium chloride)  at concentration (50%) which gave results (100%) very effective to eradication bio film ,the antibacterial activity of  thymol plant extract (carvacrol) at concentration (50%) were investigated the results proved that is effective  on disrupted biofilm in (83.4%). The used of metronidazole were very effective on biofilm (100%)as well as in Alum ,sodium chloride and sodium bicarbonate The results showed that all factors below have the ability to destruction G.vaginalis biofilm .Keywords: G. vaginalis, cpn60, 16sRNA, bio film, Preterm labor. 
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