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Gouri Kumar Dash
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Medicine & Pharmacology

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Physicochemical Analysis of Nephelium Lappaceum Seed Fat and its Application in Topical Formulations Gouri Kumar Dash
Journal of Global Pharma Technology Volume 11 Issue 07 (2019) July 2019
Publisher : Journal of Global Pharma Technology

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Abstract

Nephelium lappaceum L. (Family- Sapindaceae), popularly known as ‘Rambutan’, is well known for its edible fruits with delicious taste and flavour in Southeast Asia. The fruits are deseeded during processing in the canning industry and considered as a waste by-product. The seeds contain good amount of fatty material and has been claimed to be a source of natural edible fat. The objective of the study was to report the physicochemical parameters of the seed fat together with formulation of few topical preparations such as creams and lotions containing the seed fat that would offer new prototype formulations containing natural ingredients. The physicochemical analysis of the seed fat such as melting point, saponification value, acid value, ester value and peroxide value were studied. The GC-MS analysis of the seed fat was carried out to find its chemical composition. Further, two topical preparations such as creams and lotions were prepared using the seed fat and evaluated using standard recommended procedures. The seed fat was compatible to be used as an ingredient in the bases of creams or lotions. Keywords: N. lappaceum seed fat, Physicochemical analysis, GC-MS analysis, Topical formulations
Quantitative Estimation of Gallic Acid in Triphala Churna by HPTLC Gouri Kumar Dash
Journal of Global Pharma Technology Volume 11 Issue 03 (2019) March. 2019
Publisher : Journal of Global Pharma Technology

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Abstract

A simple, reliable and rapid HPTLC method was developed for qquantitative estimation of gallic acid in the methanol extract of Triphala churna. The method was validated as per ICH guidelines for linearity, accuracy, precision, and specificity. Silica gel 60 F254 pre-coated aluminium plates were used as the stationary phase. Toluene: Ethyl acetate: Formic acid: Methanol (12: 9: 4: 0.5) constituted the mobile phase.  Percentage of gallic acid was estimated through densitometry scanning using a TLC Scanner III (Camag, Switzerland) with winCATS software. The amount of gallic acid present in the sample was found to be 0.41 ± 0.08 % w/w with respect to dried plant material. Thus, the developed method can be applied in quantifying the amounts of gallic acid in Triphala churna and results of this work will support in the standardization process of Triphala churna using gallic acid as the biomarker.Keywords: Triphala churna, Methanol extract, HPTLC, Gallic acid.
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